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Target ID: 430745
Target Name:RF.A4044E.GBX1  Protein Accession:NM_001098834  Local Accession: RF.A4044E.GBX1  cDNA Accession:NM_001098834  PSCA PDB:2n8g

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Proposed Target  
 
Active Target  
 
PCR Testing  
 
Amplifer Generation  
 
Orientation  
 
Plasmid Purification  
 
Expression  
 
Protein Purification  
 
HSQC satisfactory  
 
[13C,15N] - labeled sample  
 
NMR Structure  
 
Refining  
 
QC  
 
PDB Deposition  
 
Proposed Target
 
Active Target
 
PCR Testing
 
Amplifer Generation
 
Orientation
 
Plasmid Purification
 
Expression
 
Protein Purification
 
HSQC satisfactory
 
[13C,15N] - labeled sample
 
NMR Structure
 
Refining
 
QC
 
PDB Deposition
 
 
Status View
 
Chrono View
 
Name: Gastrulation brain homeobox 1 protein (GBX1)  Start Position: 256  End Position: 325
Mutations: -E277R-R312E  GNF Target Alias: BL21(DE3):PET46-RF.A4044E.GBX1-HUM-363-256-325-E277R-R312E  Sequence Type: SEGMENT
Residues: 70  DNA Length: 210  Molecular Weight: 8240.17
Isoelectric Point: 10.78  Epsilon 280: 12490  Gravy Index: -0.83
Species: H. sapiens  Activated: 0  Alanine Absolute: 6
Alanine Relative: 0.0857  Arginine Absolute: 8  Arginine Relative: 0.1143
Asparagine Absolute: 2  Asparagine Relative: 0.0286  Aspartic Acid Absolute: 0
Aspartic Acid Relative: 0.0000  Cysteine Absolute: 1  Cysteine Relative: 0.0143
Glutamic Acid Absolute: 6  Glutamic Acid Relative: 0.0857  Glutamine Absolute: 4
Glutamine Relative: 0.0571  Glycine Absolute: 3  Glycine Relative: 0.0429
Histidine Absolute: 2  Histidine Relative: 0.0286  Isoleucine Absolute: 3
Isoleucine Relative: 0.0429  Leucine Absolute: 7  Leucine Relative: 0.1000
Lysine Absolute: 9  Lysine Relative: 0.1286  Methionine Absolute: 0
Methionine Relative: 0.0000  Phenylalanine Absolute: 3  Phenylalanine Relative: 0.0429
Proline Absolute: 1  Proline Relative: 0.0143  Serine Absolute: 6
Serine Relative: 0.0857  Threonine Absolute: 3  Threonine Relative: 0.0429
Tryptophan Absolute: 2  Tryptophan Relative: 0.0286  Tyrosine Absolute: 1
Tyrosine Relative: 0.0143  Valine Absolute: 3  Valine Relative: 0.0429
Project: GNF Autogenerated  Selection Date: 2011-12-02  
ProposalRelationshipOwnerLab Name
114PSI Biological PartnerFletterickPartnership for Stem Cell Biology

STAGE: Proposed Target

STAGE: Active Target

STAGE: PCR Testing

STAGE: Amplifer Generation

STAGE: Orientation

STAGE: Plasmid Purification

STAGE: Expression

STAGE: Protein Purification

STAGE: HSQC satisfactory

STAGE: [13C,15N] - labeled sample

STAGE: NMR Structure

STAGE: Refining

STAGE: QC

STAGE: PDB Deposition
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