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Target ID: 423832
Target Name:C.LS20780A  Protein Accession:NP_419126.1  Local Accession: C.LS20780A  cDNA Accession:REF|NC_002696.2|:C324036-323812  PSCA PDB:2mhe

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Proposed Target  
 
Active Target  
 
PCR Testing  
 
Amplifer Generation  
 
Orientation  
 
Plasmid Purification  
 
Micro Expression  
 
Expression  
 
Protein Purification  
 
HSQC satisfactory  
 
[13C,15N] - labeled sample  
 
Crystallization  
 
Crystal Hit  
 
SDC Receiving  
 
Screening  
 
NMR Structure  
 
Refining  
 
QC  
 
PDB Deposition  
 
Proposed Target
 
Active Target
 
PCR Testing
 
Amplifer Generation
 
Orientation
 
Plasmid Purification
 
Micro Expression
 
Expression
 
Protein Purification
 
HSQC satisfactory
 
[13C,15N] - labeled sample
 
Crystallization
 
Crystal Hit
 
SDC Receiving
 
Screening
 
NMR Structure
 
Refining
 
QC
 
PDB Deposition
 
 
Status View
 
Chrono View
 
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1 2 4 5 6 1 2 3
Name: NMR structure of DUF4170 family protein, PF13773, ClpS-like fold  Start Position: 1  End Position: 74
GNF Target Alias: PB1:SpeedET-C.LS20780A-ccr-74-1-74  Sequence Type: COMPLETE  Residues: 74
DNA Length: 222  Molecular Weight: 8229.93  Isoelectric Point: 6.16
Epsilon 280: 9970  Gravy Index: -0.30  Species: C. crescentus
Activated: 1  Alanine Absolute: 10  Alanine Relative: 0.1351
Arginine Absolute: 2  Arginine Relative: 0.0270  Asparagine Absolute: 1
Asparagine Relative: 0.0135  Aspartic Acid Absolute: 6  Aspartic Acid Relative: 0.0811
Cysteine Absolute: 0  Cysteine Relative: 0.0000  Glutamic Acid Absolute: 5
Glutamic Acid Relative: 0.0676  Glutamine Absolute: 2  Glutamine Relative: 0.0270
Glycine Absolute: 5  Glycine Relative: 0.0676  Histidine Absolute: 5
Histidine Relative: 0.0676  Isoleucine Absolute: 3  Isoleucine Relative: 0.0405
Leucine Absolute: 6  Leucine Relative: 0.0811  Lysine Absolute: 7
Lysine Relative: 0.0946  Methionine Absolute: 1  Methionine Relative: 0.0135
Phenylalanine Absolute: 3  Phenylalanine Relative: 0.0405  Proline Absolute: 3
Proline Relative: 0.0405  Serine Absolute: 3  Serine Relative: 0.0405
Threonine Absolute: 1  Threonine Relative: 0.0135  Tryptophan Absolute: 1
Tryptophan Relative: 0.0135  Tyrosine Absolute: 3  Tyrosine Relative: 0.0405
Valine Absolute: 7  Valine Relative: 0.0946  Project: Essential prots. & trans. factors, C.cr.
Selection Date: 2011-09-02  
ProposalRelationshipOwnerLab Name
108Collaborator CNT# 86ShapiroDr. Lucy Shapiro of Stanford University School of Medicine

STAGE: Proposed Target

STAGE: Active Target

STAGE: PCR Testing

STAGE: Amplifer Generation

STAGE: Orientation

STAGE: Plasmid Purification

STAGE: Micro Expression

STAGE: Expression

STAGE: Protein Purification

STAGE: HSQC satisfactory

STAGE: [13C,15N] - labeled sample

STAGE: Crystallization

STAGE: Crystal Hit

STAGE: SDC Receiving

STAGE: Screening

STAGE: NMR Structure

STAGE: Refining

STAGE: QC

STAGE: PDB Deposition
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