Target ID: | 421651 | |||||||||||||
Target Name: | WS3220C | Protein Accession: | BC040485 | Local Accession: | WS3220C.GRSF1 | cDNA Accession: | BC040485 | PSCA | PDB:4qu6 | PDB:2lmi |
Hover over nodes to see experimental data. Click on nodes to go to that point in the data below. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
Name: | G-rich RNA sequence binding factor 1, RNA binding domain, RRM_6, PF14259 family | Start Position: | 140 | End Position: | 245 | ||||||||||
GNF Target Alias: | HK100:SpeedET-WS3220C-hum-435-140-245 | Sequence Type: | SEGMENT | Residues: | 106 | ||||||||||
DNA Length: | 318 | Molecular Weight: | 12369.34 | Isoelectric Point: | 4.80 | ||||||||||
Epsilon 280: | 9970 | Gravy Index: | -0.64 | Species: | H. sapiens | ||||||||||
Activated: | 3 | Alanine Absolute: | 4 | Alanine Relative: | 0.0377 | ||||||||||
Arginine Absolute: | 8 | Arginine Relative: | 0.0755 | Asparagine Absolute: | 6 | ||||||||||
Asparagine Relative: | 0.0566 | Aspartic Acid Absolute: | 9 | Aspartic Acid Relative: | 0.0849 | ||||||||||
Cysteine Absolute: | 2 | Cysteine Relative: | 0.0189 | Glutamic Acid Absolute: | 12 | ||||||||||
Glutamic Acid Relative: | 0.1132 | Glutamine Absolute: | 5 | Glutamine Relative: | 0.0472 | ||||||||||
Glycine Absolute: | 6 | Glycine Relative: | 0.0566 | Histidine Absolute: | 2 | ||||||||||
Histidine Relative: | 0.0189 | Isoleucine Absolute: | 5 | Isoleucine Relative: | 0.0472 | ||||||||||
Leucine Absolute: | 10 | Leucine Relative: | 0.0943 | Lysine Absolute: | 6 | ||||||||||
Lysine Relative: | 0.0566 | Methionine Absolute: | 5 | Methionine Relative: | 0.0472 | ||||||||||
Phenylalanine Absolute: | 4 | Phenylalanine Relative: | 0.0377 | Proline Absolute: | 2 | ||||||||||
Proline Relative: | 0.0189 | Serine Absolute: | 7 | Serine Relative: | 0.0660 | ||||||||||
Threonine Absolute: | 1 | Threonine Relative: | 0.0094 | Tryptophan Absolute: | 1 | ||||||||||
Tryptophan Relative: | 0.0094 | Tyrosine Absolute: | 3 | Tyrosine Relative: | 0.0283 | ||||||||||
Valine Absolute: | 8 | Valine Relative: | 0.0755 | Project: | Williamson and Salomon center, set 1 | ||||||||||
Selection Date: | 2010-10-06 | ||||||||||||||
|
STAGE: Proposed Target
STAGE: Active Target
-
Plate: 108955 Edit Cell: C01 Edit Date: 2012-06-07 Status: Success
Previous Location: Plate: 0 Cell: 421651
- Sample Name: PB1:SpeedET-WS3220C-hum-435-140-245 Date: 2012-06-08
- GNF Project Name: PSIbiology-Williamson/Salomon(WS) Date: 2012-06-08
- GNF Program Name: JCSG Date: 2012-06-08
-
Plate: 25095 Edit Cell: 999-APO Edit Date: 2010-12-18 Status: Success
Previous Location: Plate: 0 Cell: 421651
- Sample Name: HEK293:pNTC7485speedEhaloT-WS3220C-hum-435-140-245 Date: 2010-12-18
- GNF Project Name: PSIbiology-Williamson/Salomon(WS) Date: 2011-10-15
- GNF Program Name: JCSG Date: 2010-12-18
- GNF Sample ID: 115476 Date: 2013-06-14
-
Plate: 25095 Edit Cell: 999-DDY Edit Date: 2013-04-09 Status: Success
Previous Location: Plate: 0 Cell: 421651
- Sample Name: BL21(DE3):SpeedE6T-WS3220C-hum-435-140-245 Date: 2013-06-25
- GNF Project Name: PSIbiology-Williamson/Salomon(WS) Date: 2013-04-09
- GNF Program Name: JCSG Date: 2013-04-09
- GNF Sample ID: 175084 Date: 2013-04-09
-
Plate: 94971 Edit Cell: B11 Edit Date: 2010-12-10 Status: Success
Previous Location: Plate: 0 Cell: 421651
- Sample Name: HK100:SpeedET-WS3220C-hum-435-140-245 Date: 2010-12-18
- GNF Project Name: PSI:Biology - Williamson/Salomon (WS) Date: 2011-03-24
- GNF Program Name: JCSG Date: 2010-12-18
-
Plate: 94972 Edit Cell: C12 Edit Date: 2010-12-14 Status: Success
Previous Location: Plate: 0 Cell: 421651
- Sample Name: HK100:pNTC7485speedEhaloT-WS3220C-hum-435-140-245 Date: 2010-12-18
- GNF Project Name: PSI:Biology - Williamson/Salomon (WS) Date: 2011-03-24
- GNF Program Name: JCSG Date: 2010-12-18
STAGE: PCR Testing
-
Plate: 108955 Edit Cell: C01 Edit Date: 2012-06-07 Status: Success
Previous Location: Plate: 108955 Cell: C01
-
Plate: 25095 Edit Cell: 999-APO Edit Date: 2010-12-18 Status: Success
Previous Location: Plate: 25095 Cell: 999-APO
-
Plate: 25095 Edit Cell: 999-DDY Edit Date: 2013-04-09 Status: Success
Previous Location: Plate: 25095 Cell: 999-DDY
-
Plate: 94971 Edit Cell: B11 Edit Date: 2010-12-10 Status: Success
Previous Location: Plate: 94971 Cell: B11
-
Plate: 94972 Edit Cell: C12 Edit Date: 2010-12-14 Status: Success
Previous Location: Plate: 94972 Cell: C12
STAGE: Amplifer Generation
-
Plate: 108955 Edit Cell: C01 Edit Date: 2012-06-07 Status: Success
Previous Location: Plate: 108955 Cell: C01
-
Plate: 25095 Edit Cell: 999-APO Edit Date: 2010-12-18 Status: Success
Previous Location: Plate: 25095 Cell: 999-APO
-
Plate: 25095 Edit Cell: 999-DDY Edit Date: 2013-04-09 Status: Success
Previous Location: Plate: 25095 Cell: 999-DDY
-
Plate: 94971 Edit Cell: B11 Edit Date: 2010-12-10 Status: Success
Previous Location: Plate: 94971 Cell: B11
-
Plate: 94972 Edit Cell: C12 Edit Date: 2010-12-14 Status: Success
Previous Location: Plate: 94972 Cell: C12
STAGE: Orientation
-
Plate: 108955 Edit Cell: C01 Edit Date: 2012-06-07 Status: Success
Previous Location: Plate: 108955 Cell: C01
-
Plate: 25095 Edit Cell: 999-APO Edit Date: 2010-12-18 Status: Success
Previous Location: Plate: 25095 Cell: 999-APO
-
Plate: 25095 Edit Cell: 999-DDY Edit Date: 2013-04-09 Status: Success
Previous Location: Plate: 25095 Cell: 999-DDY
-
Plate: 94971 Edit Cell: B11 Edit Date: 2010-12-10 Status: Success
Previous Location: Plate: 94971 Cell: B11
-
Plate: 94972 Edit Cell: C12 Edit Date: 2010-12-14 Status: Success
Previous Location: Plate: 94972 Cell: C12
STAGE: Plasmid Purification
-
Plate: 108955 Edit (C-1076) Cell: C01 Edit Date: 2012-06-07 Status: Success
Previous Location: Plate: 108955 (C-1076) Cell: C01
- Clone ID: 108348 Date: 2012-06-08
- Strain/Vector: PB1 Date: 2012-06-08
- Clone Site: SpeedET Date: 2012-06-08
- Cloned Date: 2012-06-07 Date: 2012-07-07
-
Plate: 25095 Edit (C-9999) Cell: 999-APO Edit Date: 2010-12-18 Status: Success
Previous Location: Plate: 25095 (C-9999) Cell: 999-APO
- Clone ID: 85982 Date: 2010-12-18
- Strain/Vector: HEK293 Date: 2010-12-18
- Clone Site: pNTC7485speedEhaloT Date: 2010-12-18
-
Plate: 25095 Edit (C-9999) Cell: 999-DDY Edit Date: 2013-04-09 Status: Success
Previous Location: Plate: 25095 (C-9999) Cell: 999-DDY
- Clone ID: 125797 Date: 2013-04-09
- Strain/Vector: BL21(DE3) Date: 2013-04-09
- Clone Site: SpeedE6T Date: 2013-04-09
-
Plate: 94971 Edit (C-0917) Cell: B11 Edit Date: 2010-12-10 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Clone ID: 85779 Date: 2010-12-18
- Strain/Vector: HK100 Date: 2010-12-18
- Clone Site: SpeedET Date: 2010-12-18
- Cloned Date: 2010-12-10 Date: 2011-03-24
-
Plate: 94972 Edit (C-0918) Cell: C12 Edit Date: 2010-12-14 Status: Success
Previous Location: Plate: 94972 (C-0918) Cell: C12
- Clone ID: 85887 Date: 2010-12-18
- Strain/Vector: HK100 Date: 2010-12-18
- Clone Site: pNTC7485speedEhaloT Date: 2010-12-18
- Cloned Date: 2010-12-14 Date: 2011-03-24
STAGE: Expression
-
Plate: 109001 Edit (E-2517) Cell: C01 Edit Date: 2012-06-08 Status: Success
Previous Location: Plate: 108955 (C-1076) Cell: C01
- Fermentation Date: 2012-06-08 Date: 2012-06-14
- Protein ID: 369654 Date: 2012-06-09
- Media: Native-TB Date: 2012-06-09
-
Plate: 116030 Edit Cell: NMR Edit Date: 2011-12-01 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Protein ID: NMRPID_421651 Date: 2013-04-08
-
Plate: 127645 Edit (E-2841) Cell: D01 Edit Date: 2016-03-17 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2016-03-17 Date: 2016-04-02
- Protein ID: 431814 Date: 2016-04-02
- Media: Native-TB Date: 2016-04-02
-
Plate: 25158 Edit (Expression Attempt) Cell: 999-NL Edit Date: 2012-11-21 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Comment: RNA-12b:GGAGGGUGUGGG, Buffer:20mM Tris-HCl, pH 7.0, 50mM NaCl, 1 mM DTT Date: 2012-11-21
- Fermentation Date: 2015-06-17 Date: 2016-04-03
- Protein ID: 379060 Date: 2012-11-21
- Media: Native-TB Date: 2012-11-21
-
Plate: 25158 Edit (Expression Attempt) Cell: 999-OT Edit Date: 2013-04-09 Status: Success
Previous Location: Plate: 25095 (C-9999) Cell: 999-DDY
- Comment: cleaved, RNA-6b:AGGGUA Date: 2013-04-09
- Fermentation Date: 2015-06-17 Date: 2016-04-03
- Protein ID: 387766 Date: 2013-04-09
- Media: Native-TB Date: 2013-04-09
-
Plate: 25158 Edit (Expression Attempt) Cell: 999-OU Edit Date: 2013-04-09 Status: Success
Previous Location: Plate: 25095 (C-9999) Cell: 999-DDY
- Comment: cleaved, RNA-6b:AGGGUG Date: 2013-04-09
- Fermentation Date: 2015-06-17 Date: 2016-04-03
- Protein ID: 387767 Date: 2013-04-09
- Media: Native-TB Date: 2013-04-09
-
Plate: 25158 Edit (Expression Attempt) Cell: 999-PU Edit Date: 2013-07-30 Status: Success
Previous Location: Plate: 25095 (C-9999) Cell: 999-DDY
- Comment: Complex with RNA 6b:AGGGAU Date: 2013-07-30
- Fermentation Date: 2015-06-17 Date: 2016-04-03
- Protein ID: 399455 Date: 2013-07-30
- Media: Native-TB Date: 2013-07-30
-
Plate: 95009 Edit (E-2212) Cell: A07 Edit Date: 2011-01-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-01-11 Date: 2012-02-26
- GNFuge Date: 2011-01-17 Date: 2012-02-26
- Protein ID: 322288 Date: 2011-01-14
- Media: Met-Inhibition Date: 2011-01-14
-
Plate: 95009 Edit (E-2212) Cell: B07 Edit Date: 2011-01-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-01-11 Date: 2012-02-26
- GNFuge Date: 2011-01-17 Date: 2012-02-26
- Protein ID: 322300 Date: 2011-01-14
- Media: Met-Inhibition Date: 2011-01-14
-
Plate: 95009 Edit (E-2212) Cell: C07 Edit Date: 2011-01-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-01-11 Date: 2012-02-26
- GNFuge Date: 2011-01-17 Date: 2012-02-26
- Protein ID: 322312 Date: 2011-01-14
- Media: Met-Inhibition Date: 2011-01-14
-
Plate: 95009 Edit (E-2212) Cell: D07 Edit Date: 2011-01-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-01-11 Date: 2012-02-26
- GNFuge Date: 2011-01-17 Date: 2012-02-26
- Protein ID: 322324 Date: 2011-01-14
- Media: Met-Inhibition Date: 2011-01-14
-
Plate: 95009 Edit (E-2212) Cell: E07 Edit Date: 2011-01-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-01-11 Date: 2012-02-26
- GNFuge Date: 2011-01-17 Date: 2012-02-26
- Protein ID: 322336 Date: 2011-01-14
- Media: Met-Inhibition Date: 2011-01-14
-
Plate: 95009 Edit (E-2212) Cell: F07 Edit Date: 2011-01-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-01-11 Date: 2012-02-26
- GNFuge Date: 2011-01-17 Date: 2012-02-26
- Protein ID: 322348 Date: 2011-01-14
- Media: Met-Inhibition Date: 2011-01-14
-
Plate: 95009 Edit (E-2212) Cell: G07 Edit Date: 2011-01-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-01-11 Date: 2012-02-26
- GNFuge Date: 2011-01-17 Date: 2012-02-26
- Protein ID: 322360 Date: 2011-01-14
- Media: Met-Inhibition Date: 2011-01-14
-
Plate: 95009 Edit (E-2212) Cell: H07 Edit Date: 2011-01-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-01-11 Date: 2012-02-26
- GNFuge Date: 2011-01-17 Date: 2012-02-26
- Protein ID: 322372 Date: 2011-01-14
- Media: Met-Inhibition Date: 2011-01-14
-
Plate: 95286 Edit (P-0325) Cell: H07 Edit Date: 2011-02-03 Status: Success
Previous Location: Plate: 25095 (C-9999) Cell: 999-APO
- Fermentation Date: 2011-02-03 Date: 2012-04-22
- Protein ID: 324390 Date: 2011-02-05
- Media: Freestyle Date: 2011-02-05
-
Plate: 97179 Edit (E-2292) Cell: A07 Edit Date: 2011-05-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-05-11 Date: 2011-08-21
- GNFuge Date: 2011-05-25 Date: 2011-08-21
- Protein ID: 334362 Date: 2011-05-13
- Media: Met-Inhibition Date: 2011-05-13
-
Plate: 97179 Edit (E-2292) Cell: B07 Edit Date: 2011-05-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-05-11 Date: 2011-08-21
- GNFuge Date: 2011-05-25 Date: 2011-08-21
- Protein ID: 334374 Date: 2011-05-13
- Media: Met-Inhibition Date: 2011-05-13
-
Plate: 97179 Edit (E-2292) Cell: C07 Edit Date: 2011-05-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-05-11 Date: 2011-08-21
- GNFuge Date: 2011-05-25 Date: 2011-08-21
- Protein ID: 334386 Date: 2011-05-13
- Media: Met-Inhibition Date: 2011-05-13
-
Plate: 97179 Edit (E-2292) Cell: D07 Edit Date: 2011-05-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-05-11 Date: 2011-08-21
- GNFuge Date: 2011-05-25 Date: 2011-08-21
- Protein ID: 334398 Date: 2011-05-13
- Media: Met-Inhibition Date: 2011-05-13
-
Plate: 97179 Edit (E-2292) Cell: E07 Edit Date: 2011-05-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-05-11 Date: 2011-08-21
- GNFuge Date: 2011-05-25 Date: 2011-08-21
- Protein ID: 334410 Date: 2011-05-13
- Media: Met-Inhibition Date: 2011-05-13
-
Plate: 97179 Edit (E-2292) Cell: F07 Edit Date: 2011-05-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-05-11 Date: 2011-08-21
- GNFuge Date: 2011-05-25 Date: 2011-08-21
- Protein ID: 334422 Date: 2011-05-13
- Media: Met-Inhibition Date: 2011-05-13
-
Plate: 97179 Edit (E-2292) Cell: G07 Edit Date: 2011-05-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-05-11 Date: 2011-08-21
- GNFuge Date: 2011-05-25 Date: 2011-08-21
- Protein ID: 334434 Date: 2011-05-13
- Media: Met-Inhibition Date: 2011-05-13
-
Plate: 97179 Edit (E-2292) Cell: H07 Edit Date: 2011-05-11 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-05-11 Date: 2011-08-21
- GNFuge Date: 2011-05-25 Date: 2011-08-21
- Protein ID: 334446 Date: 2011-05-13
- Media: Met-Inhibition Date: 2011-05-13
-
Plate: 97973 Edit (E-2311) Cell: A11 Edit Date: 2011-06-09 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-06-09 Date: 2011-12-12
- Protein ID: 337016 Date: 2011-06-11
- Media: Met-Inhibition Date: 2011-06-11
-
Plate: 97973 Edit (E-2311) Cell: B11 Edit Date: 2011-06-09 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-06-09 Date: 2011-12-12
- Protein ID: 337028 Date: 2011-06-11
- Media: Met-Inhibition Date: 2011-06-11
-
Plate: 97973 Edit (E-2311) Cell: C11 Edit Date: 2011-06-09 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-06-09 Date: 2011-12-12
- Protein ID: 337040 Date: 2011-06-11
- Media: Met-Inhibition Date: 2011-06-11
-
Plate: 97973 Edit (E-2311) Cell: D11 Edit Date: 2011-06-09 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-06-09 Date: 2011-12-12
- Protein ID: 337052 Date: 2011-06-11
- Media: Met-Inhibition Date: 2011-06-11
-
Plate: 97973 Edit (E-2311) Cell: E11 Edit Date: 2011-06-09 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-06-09 Date: 2011-12-12
- Protein ID: 337064 Date: 2011-06-11
- Media: Met-Inhibition Date: 2011-06-11
-
Plate: 97973 Edit (E-2311) Cell: F11 Edit Date: 2011-06-09 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-06-09 Date: 2011-12-12
- Protein ID: 337076 Date: 2011-06-11
- Media: Met-Inhibition Date: 2011-06-11
-
Plate: 97973 Edit (E-2311) Cell: G11 Edit Date: 2011-06-09 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-06-09 Date: 2011-12-12
- Protein ID: 337088 Date: 2011-06-11
- Media: Met-Inhibition Date: 2011-06-11
-
Plate: 97973 Edit (E-2311) Cell: H11 Edit Date: 2011-06-09 Status: Success
Previous Location: Plate: 94971 (C-0917) Cell: B11
- Fermentation Date: 2011-06-09 Date: 2011-12-12
- Protein ID: 337100 Date: 2011-06-11
- Media: Met-Inhibition Date: 2011-06-11
STAGE: Protein Purification
-
Plate: 109001 Edit (E-2517) Cell: C01 Edit Date: 2012-06-09 Status: Success
Previous Location: Plate: 109001 (E-2517) Cell: C01
-
Plate: 116127 Edit Cell: NMR Edit Date: 2011-12-01 Status: Success
Previous Location: Plate: 116030 Cell: NMR
-
Plate: 127645 Edit (E-2841) Cell: D01 Edit Date: 2016-04-02 Status: Success
Previous Location: Plate: 127645 (E-2841) Cell: D01
-
Plate: 25158 Edit (Expression Attempt) Cell: 999-NL Edit Date: 2012-11-21 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL
- Purified Concentration (mg/mL): 10.0 Date: 2012-11-21
-
Plate: 25158 Edit (Expression Attempt) Cell: 999-OT Edit Date: 2013-04-09 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
-
Plate: 25158 Edit (Expression Attempt) Cell: 999-OU Edit Date: 2013-04-09 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU
-
Plate: 25158 Edit (Expression Attempt) Cell: 999-PU Edit Date: 2013-07-30 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-PU
-
Plate: 95009 Edit (E-2212) Cell: A07 Edit Date: 2011-01-14 Status: Success
Previous Location: Plate: 95009 (E-2212) Cell: A07
- Purified Concentration (mg/mL): 20.0 Date: 2011-01-27
-
Plate: 95009 Edit (E-2212) Cell: B07 Edit Date: 2011-01-14 Status: Success
Previous Location: Plate: 95009 (E-2212) Cell: B07
-
Plate: 95009 Edit (E-2212) Cell: C07 Edit Date: 2011-01-14 Status: Success
Previous Location: Plate: 95009 (E-2212) Cell: C07
-
Plate: 95009 Edit (E-2212) Cell: D07 Edit Date: 2011-01-14 Status: Success
Previous Location: Plate: 95009 (E-2212) Cell: D07
-
Plate: 95009 Edit (E-2212) Cell: E07 Edit Date: 2011-01-14 Status: Success
Previous Location: Plate: 95009 (E-2212) Cell: E07
-
Plate: 95009 Edit (E-2212) Cell: F07 Edit Date: 2011-01-14 Status: Success
Previous Location: Plate: 95009 (E-2212) Cell: F07
-
Plate: 95009 Edit (E-2212) Cell: G07 Edit Date: 2011-01-14 Status: Success
Previous Location: Plate: 95009 (E-2212) Cell: G07
-
Plate: 95009 Edit (E-2212) Cell: H07 Edit Date: 2011-01-14 Status: Success
Previous Location: Plate: 95009 (E-2212) Cell: H07
-
Plate: 95286 Edit (P-0325) Cell: H07 Edit Date: 2011-02-05 Status: Success
Previous Location: Plate: 95286 (P-0325) Cell: H07
-
Plate: 97179 Edit (E-2292) Cell: A07 Edit Date: 2011-05-13 Status: Success
Previous Location: Plate: 97179 (E-2292) Cell: A07
- Purified Concentration (mg/mL): 14.2 Date: 2011-06-05
-
Plate: 97179 Edit (E-2292) Cell: B07 Edit Date: 2011-05-13 Status: Success
Previous Location: Plate: 97179 (E-2292) Cell: B07
-
Plate: 97179 Edit (E-2292) Cell: C07 Edit Date: 2011-05-13 Status: Success
Previous Location: Plate: 97179 (E-2292) Cell: C07
-
Plate: 97179 Edit (E-2292) Cell: D07 Edit Date: 2011-05-13 Status: Success
Previous Location: Plate: 97179 (E-2292) Cell: D07
-
Plate: 97179 Edit (E-2292) Cell: E07 Edit Date: 2011-05-13 Status: Success
Previous Location: Plate: 97179 (E-2292) Cell: E07
-
Plate: 97179 Edit (E-2292) Cell: F07 Edit Date: 2011-05-13 Status: Success
Previous Location: Plate: 97179 (E-2292) Cell: F07
-
Plate: 97179 Edit (E-2292) Cell: G07 Edit Date: 2011-05-13 Status: Success
Previous Location: Plate: 97179 (E-2292) Cell: G07
-
Plate: 97179 Edit (E-2292) Cell: H07 Edit Date: 2011-05-13 Status: Success
Previous Location: Plate: 97179 (E-2292) Cell: H07
-
Plate: 97973 Edit (E-2311) Cell: A11 Edit Date: 2011-06-11 Status: Success
Previous Location: Plate: 97973 (E-2311) Cell: A11
-
Plate: 97973 Edit (E-2311) Cell: B11 Edit Date: 2011-06-11 Status: Success
Previous Location: Plate: 97973 (E-2311) Cell: B11
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Plate: 97973 Edit (E-2311) Cell: C11 Edit Date: 2011-06-11 Status: Success
Previous Location: Plate: 97973 (E-2311) Cell: C11
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Plate: 97973 Edit (E-2311) Cell: D11 Edit Date: 2011-06-11 Status: Success
Previous Location: Plate: 97973 (E-2311) Cell: D11
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Plate: 97973 Edit (E-2311) Cell: E11 Edit Date: 2011-06-11 Status: Success
Previous Location: Plate: 97973 (E-2311) Cell: E11
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Plate: 97973 Edit (E-2311) Cell: F11 Edit Date: 2011-06-11 Status: Success
Previous Location: Plate: 97973 (E-2311) Cell: F11
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Plate: 97973 Edit (E-2311) Cell: G11 Edit Date: 2011-06-11 Status: Success
Previous Location: Plate: 97973 (E-2311) Cell: G11
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Plate: 97973 Edit (E-2311) Cell: H11 Edit Date: 2011-06-11 Status: Success
Previous Location: Plate: 97973 (E-2311) Cell: H11
STAGE: HSQC satisfactory
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NMR Tube: 116239 Edit Cell: NMR Edit Date: 2011-12-01 Status: In Progress
Previous Location: Plate: 116127 Cell: NMR
STAGE: [13C,15N] - labeled sample
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NMR Tube: 116280 Edit Cell: NMR Edit Date: 2011-12-01 Status: In Progress
Previous Location: NMR Tube: 116239 Cell: NMR
STAGE: Crystallization
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Plate: 112901 Edit (UZ004652) Cell: XT Edit Date: 2012-11-21 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL
- Screen Type: Sparse Matrix Date: 2012-12-30
- Setup Date: 2012-11-21 Date: 2012-11-22
- Setup Temp (C): 4 Date: 2012-11-22
- Crystal Conditions: The JCSG Core Suite I Date: 2012-12-30
- Drop Volume (nl): 200 Date: 2012-12-30
- Reservoir Volume (ul): 35 Date: 2012-12-30
- CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30
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Plate: 112904 Edit (UZ004655) Cell: XT Edit Date: 2012-11-21 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL
- Screen Type: Sparse Matrix Date: 2012-12-30
- Setup Date: 2012-11-21 Date: 2012-11-22
- Setup Temp (C): 4 Date: 2012-11-22
- Crystal Conditions: The JCSG Core Suite II Date: 2012-12-30
- Drop Volume (nl): 200 Date: 2012-12-30
- Reservoir Volume (ul): 35 Date: 2012-12-30
- CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30
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Plate: 112907 Edit (UZ004658) Cell: XT Edit Date: 2012-11-21 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL
- Screen Type: Sparse Matrix Date: 2012-12-30
- Setup Date: 2012-11-21 Date: 2012-11-22
- Setup Temp (C): 4 Date: 2012-11-22
- Crystal Conditions: The JCSG Core Suite III Date: 2012-12-30
- Drop Volume (nl): 200 Date: 2012-12-30
- Reservoir Volume (ul): 35 Date: 2012-12-30
- CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30
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Plate: 112910 Edit (UZ004661) Cell: XT Edit Date: 2012-11-21 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL
- Screen Type: Sparse Matrix Date: 2012-12-30
- Setup Date: 2012-11-21 Date: 2012-11-22
- Setup Temp (C): 4 Date: 2012-11-22
- Crystal Conditions: The JCSG Core Suite IV Date: 2012-12-30
- Drop Volume (nl): 200 Date: 2012-12-30
- Reservoir Volume (ul): 35 Date: 2012-12-30
- CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30
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Plate: 113069 Edit (S0035613) Cell: XT Edit Date: 2012-11-29 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL
- Screen Type: Sparse Matrix Date: 2012-12-30
- Setup Date: 2012-11-29 Date: 2012-11-30
- Setup Temp (C): 4 Date: 2012-11-30
- Crystal Conditions: The Nucleix Suite Date: 2012-12-30
- Drop Volume (nl): 200 Date: 2012-12-30
- Reservoir Volume (ul): 50 Date: 2012-12-30
- CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30
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Plate: 113070 Edit (S0035615) Cell: XT Edit Date: 2012-11-29 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL
- Screen Type: Sparse Matrix Date: 2012-12-30
- Setup Date: 2012-11-29 Date: 2012-11-30
- Setup Temp (C): 4 Date: 2012-11-30
- Crystal Conditions: The Protein Complex Suite Date: 2012-12-30
- Drop Volume (nl): 200 Date: 2012-12-30
- Reservoir Volume (ul): 50 Date: 2012-12-30
- CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30
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Plate: 116426 Edit (UZ007080) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 20 Date: 2013-04-14
- Crystal Conditions: The Protein Complex Suite Date: 2014-05-22
- Drop Volume (nl): 200 Date: 2014-05-22
- Reservoir Volume (ul): 35 Date: 2014-05-22
- CoCrystal Compound: 1mM RNA-6b:AGGGUA Date: 2014-05-22
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Plate: 116427 Edit (UZ007082) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 4 Date: 2013-04-14
- Crystal Conditions: GNF1 Date: 2014-05-30
- Drop Volume (nl): 100.0 Date: 2014-05-30
- Precipitate Volume (nl): 100 Date: 2014-05-30
- Reservoir Volume (ul): 50.0 Date: 2014-05-30
- CoCrystal Compound: RNA-6b:AGGGUA 1 mM Date: 2014-05-30
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Plate: 116428 Edit (UZ007083) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 20 Date: 2013-04-14
- Crystal Conditions: GNF1 Date: 2014-05-30
- Drop Volume (nl): 100.0 Date: 2014-05-30
- Precipitate Volume (nl): 100 Date: 2014-05-30
- Reservoir Volume (ul): 50.0 Date: 2014-05-30
- CoCrystal Compound: RNA-6b:AGGGUA 1 mM Date: 2014-05-30
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Plate: 116429 Edit (UZ007084) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 4 Date: 2013-04-14
- Crystal Conditions: Top96 Date: 2013-04-14
- Drop Volume (nl): 200 Date: 2014-05-22
- Reservoir Volume (ul): 35 Date: 2014-05-22
- CoCrystal Compound: 1mM RNA-6b:AGGGUG Date: 2014-05-22
-
Plate: 116430 Edit (UZ007085) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 20 Date: 2013-04-14
- Crystal Conditions: Top96 Date: 2013-04-14
- Drop Volume (nl): 200 Date: 2014-05-22
- Reservoir Volume (ul): 35 Date: 2014-05-22
- CoCrystal Compound: 1mM RNA-6b:AGGGUG Date: 2014-05-22
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Plate: 116431 Edit (UZ007086) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 4 Date: 2013-04-14
- Crystal Conditions: Top96 Date: 2013-04-14
- Drop Volume (nl): 200 Date: 2014-05-22
- Reservoir Volume (ul): 35 Date: 2014-05-22
- CoCrystal Compound: 1mM RNA-6b:AGGGUA Date: 2014-05-22
-
Plate: 116432 Edit (UZ007087) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 20 Date: 2013-04-14
- Crystal Conditions: Top96 Date: 2013-04-14
- Drop Volume (nl): 200 Date: 2014-05-22
- Reservoir Volume (ul): 35 Date: 2014-05-22
- CoCrystal Compound: 1mM RNA-6b:AGGGUA Date: 2014-05-22
-
Plate: 116433 Edit (UZ007089) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
- Screen Type: Fine Date: 2013-04-14
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 20 Date: 2013-04-14
- Crystal Conditions: UZ007089-NatrixHT Date: 2014-05-24
- Drop Volume (nl): 100.0 Date: 2014-05-24
- Precipitate Volume (nl): 100 Date: 2014-05-24
- Reservoir Volume (ul): 50.0 Date: 2014-05-24
- CoCrystal Compound: RNA-6b:AGGGUA 1 mM Date: 2014-05-24
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Plate: 116434 Edit (UZ007090) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 4 Date: 2013-04-14
- Crystal Conditions: The Protein Complex Suite Date: 2014-05-22
- Drop Volume (nl): 200 Date: 2014-05-22
- Reservoir Volume (ul): 35 Date: 2014-05-22
- CoCrystal Compound: 1mM RNA-6b:AGGGUG Date: 2014-05-22
-
Plate: 116435 Edit (UZ007091) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 20 Date: 2013-04-14
- Crystal Conditions: The Protein Complex Suite Date: 2014-05-22
- Drop Volume (nl): 200 Date: 2014-05-22
- Reservoir Volume (ul): 35 Date: 2014-05-22
- CoCrystal Compound: 1mM RNA-6b:AGGGUG Date: 2014-05-22
-
Plate: 116436 Edit (UZ007092) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 4 Date: 2013-04-14
- Crystal Conditions: The Protein Complex Suite Date: 2014-05-22
- Drop Volume (nl): 200 Date: 2014-05-22
- Reservoir Volume (ul): 35 Date: 2014-05-22
- CoCrystal Compound: 1mM RNA-6b:AGGGUA Date: 2014-05-22
-
Plate: 116437 Edit (UZ007093) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 4 Date: 2013-04-14
- Crystal Conditions: GNF1 Date: 2014-05-30
- Drop Volume (nl): 100.0 Date: 2014-05-30
- Precipitate Volume (nl): 100 Date: 2014-05-30
- Reservoir Volume (ul): 50.0 Date: 2014-05-30
- CoCrystal Compound: RNA-6b:AGGGUG 1 mM Date: 2014-05-30
-
Plate: 116438 Edit (UZ007094) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU
- Screen Type: Sparse Matrix Date: 2014-05-22
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 20 Date: 2013-04-14
- Crystal Conditions: GNF1 Date: 2014-05-30
- Drop Volume (nl): 100.0 Date: 2014-05-30
- Precipitate Volume (nl): 100 Date: 2014-05-30
- Reservoir Volume (ul): 50.0 Date: 2014-05-30
- CoCrystal Compound: RNA-6b:AGGGUG 1 mM Date: 2014-05-30
-
Plate: 116439 Edit (UZ007096) Cell: XT Edit Date: 2013-04-13 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
- Screen Type: Fine Date: 2013-04-14
- Setup Date: 2013-04-13 Date: 2013-04-14
- Setup Temp (C): 4 Date: 2013-04-14
- Crystal Conditions: UZ007096-NatrixHT Date: 2014-05-24
- Drop Volume (nl): 100.0 Date: 2014-05-24
- Precipitate Volume (nl): 100 Date: 2014-05-24
- Reservoir Volume (ul): 50.0 Date: 2014-05-24
- CoCrystal Compound: RNA-6b:AGGGUA 1 mM Date: 2014-05-24
-
Plate: 116455 Edit (UZ007148) Cell: XT Edit Date: 2013-04-15 Status: Success
Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU
- Screen Type: Fine Date: 2013-04-16
- Setup Date: 2013-04-15 Date: 2013-04-16
- Setup Temp (C): 20 Date: 2013-04-16
- Crystal Conditions: UZ007148-NatrixHT Date: 2014-05-24
- Drop Volume (nl): 100.0 Date: 2014-05-24
- Precipitate Volume (nl): 100 Date: 2014-05-24
- Reservoir Volume (ul): 50.0 Date: 2014-05-24
- CoCrystal Compound: RNA-6b:AGGGUG 1 mM Date: 2014-05-24
- Plate: 95390 Edit