JCSG Target History

Target ID:	421651
Target Name:	WS3220C		Protein Accession:	BC040485		Local Accession:	WS3220C.GRSF1		cDNA Accession:	BC040485		PSCA	PDB:4qu6	PDB:2lmi

Target ID:

421651

Target Name:

WS3220C

Protein Accession:

BC040485

Local Accession:

WS3220C.GRSF1

cDNA Accession:

BC040485

PSCA

PDB:4qu6

PDB:2lmi

Hover over nodes to see experimental data. Click on nodes to go to that point in the data below.

Proposed Target

Active Target

PCR Testing

Amplifer Generation

Orientation

Plasmid Purification

Expression

Protein Purification

HSQC satisfactory

[13C,15N] - labeled sample

Crystallization

Crystal Hit

SDC Receiving

Screening

Data Collection

Integration

Scaling

Truncating

Phasing

Tracing

MR Tracing

NMR Structure

Refining

Validation

PDB Deposition

Proposed Target

Active Target

PCR Testing

Amplifer Generation

Orientation

Plasmid Purification

Expression

Protein Purification

HSQC satisfactory

[13C,15N] - labeled sample

Crystallization

Crystal Hit

SDC Receiving

Screening

Data Collection

Integration

Scaling

Truncating

Phasing

Tracing

MR Tracing

NMR Structure

Refining

Validation

PDB Deposition

Status View


Chrono View

Name:

G-rich RNA sequence binding factor 1, RNA binding domain, RRM_6, PF14259 family

Start Position:

140

End Position:

245

GNF Target Alias:

HK100:SpeedET-WS3220C-hum-435-140-245

Sequence Type:

SEGMENT

Residues:

106

DNA Length:

318

Molecular Weight:

12369.34

Isoelectric Point:

4.80

Epsilon 280:

9970

Gravy Index:

-0.64

Species:

H. sapiens

Activated:

Alanine Absolute:

Alanine Relative:

0.0377

Arginine Absolute:

Arginine Relative:

0.0755

Asparagine Absolute:

Asparagine Relative:

0.0566

Aspartic Acid Absolute:

Aspartic Acid Relative:

0.0849

Cysteine Absolute:

Cysteine Relative:

0.0189

Glutamic Acid Absolute:

Glutamic Acid Relative:

0.1132

Glutamine Absolute:

Glutamine Relative:

0.0472

Glycine Absolute:

Glycine Relative:

0.0566

Histidine Absolute:

Histidine Relative:

0.0189

Isoleucine Absolute:

Isoleucine Relative:

0.0472

Leucine Absolute:

Leucine Relative:

0.0943

Lysine Absolute:

Lysine Relative:

0.0566

Methionine Absolute:

Methionine Relative:

0.0472

Phenylalanine Absolute:

Phenylalanine Relative:

0.0377

Proline Absolute:

Proline Relative:

0.0189

Serine Absolute:

Serine Relative:

0.0660

Threonine Absolute:

Threonine Relative:

0.0094

Tryptophan Absolute:

Tryptophan Relative:

0.0094

Tyrosine Absolute:

Tyrosine Relative:

0.0283

Valine Absolute:

Valine Relative:

0.0755

Project:

Williamson and Salomon center, set 1

Selection Date:

2010-10-06

Proposal	Relationship	Owner	Lab Name
93	PSI Biological Partner	Williamson and Salomon	Partnership for T-Cell Biology

STAGE: Proposed Target

Plate: 0 Edit Cell: 421651 Edit Date: 2010-10-06 Status: Success

STAGE: Active Target

Plate: 108955 Edit Cell: C01 Edit Date: 2012-06-07 Status: Success Previous Location: Plate: 0 Cell: 421651

Sample Name: PB1:SpeedET-WS3220C-hum-435-140-245 Date: 2012-06-08
GNF Project Name: PSIbiology-Williamson/Salomon(WS) Date: 2012-06-08
GNF Program Name: JCSG Date: 2012-06-08

Plate: 25095 Edit Cell: 999-APO Edit Date: 2010-12-18 Status: Success Previous Location: Plate: 0 Cell: 421651

Sample Name: HEK293:pNTC7485speedEhaloT-WS3220C-hum-435-140-245 Date: 2010-12-18
GNF Project Name: PSIbiology-Williamson/Salomon(WS) Date: 2011-10-15
GNF Program Name: JCSG Date: 2010-12-18
GNF Sample ID: 115476 Date: 2013-06-14

Plate: 25095 Edit Cell: 999-DDY Edit Date: 2013-04-09 Status: Success Previous Location: Plate: 0 Cell: 421651

Sample Name: BL21(DE3):SpeedE6T-WS3220C-hum-435-140-245 Date: 2013-06-25
GNF Project Name: PSIbiology-Williamson/Salomon(WS) Date: 2013-04-09
GNF Program Name: JCSG Date: 2013-04-09
GNF Sample ID: 175084 Date: 2013-04-09

Plate: 94971 Edit Cell: B11 Edit Date: 2010-12-10 Status: Success Previous Location: Plate: 0 Cell: 421651

Sample Name: HK100:SpeedET-WS3220C-hum-435-140-245 Date: 2010-12-18
GNF Project Name: PSI:Biology - Williamson/Salomon (WS) Date: 2011-03-24
GNF Program Name: JCSG Date: 2010-12-18

Plate: 94972 Edit Cell: C12 Edit Date: 2010-12-14 Status: Success Previous Location: Plate: 0 Cell: 421651

Sample Name: HK100:pNTC7485speedEhaloT-WS3220C-hum-435-140-245 Date: 2010-12-18
GNF Project Name: PSI:Biology - Williamson/Salomon (WS) Date: 2011-03-24
GNF Program Name: JCSG Date: 2010-12-18

STAGE: PCR Testing

Plate: 108955 Edit Cell: C01 Edit Date: 2012-06-07 Status: Success Previous Location: Plate: 108955 Cell: C01
Plate: 25095 Edit Cell: 999-APO Edit Date: 2010-12-18 Status: Success Previous Location: Plate: 25095 Cell: 999-APO
Plate: 25095 Edit Cell: 999-DDY Edit Date: 2013-04-09 Status: Success Previous Location: Plate: 25095 Cell: 999-DDY
Plate: 94971 Edit Cell: B11 Edit Date: 2010-12-10 Status: Success Previous Location: Plate: 94971 Cell: B11
Plate: 94972 Edit Cell: C12 Edit Date: 2010-12-14 Status: Success Previous Location: Plate: 94972 Cell: C12

STAGE: Amplifer Generation

Plate: 108955 Edit Cell: C01 Edit Date: 2012-06-07 Status: Success Previous Location: Plate: 108955 Cell: C01
Plate: 25095 Edit Cell: 999-APO Edit Date: 2010-12-18 Status: Success Previous Location: Plate: 25095 Cell: 999-APO
Plate: 25095 Edit Cell: 999-DDY Edit Date: 2013-04-09 Status: Success Previous Location: Plate: 25095 Cell: 999-DDY
Plate: 94971 Edit Cell: B11 Edit Date: 2010-12-10 Status: Success Previous Location: Plate: 94971 Cell: B11
Plate: 94972 Edit Cell: C12 Edit Date: 2010-12-14 Status: Success Previous Location: Plate: 94972 Cell: C12

STAGE: Orientation

Plate: 108955 Edit Cell: C01 Edit Date: 2012-06-07 Status: Success Previous Location: Plate: 108955 Cell: C01
Plate: 25095 Edit Cell: 999-APO Edit Date: 2010-12-18 Status: Success Previous Location: Plate: 25095 Cell: 999-APO
Plate: 25095 Edit Cell: 999-DDY Edit Date: 2013-04-09 Status: Success Previous Location: Plate: 25095 Cell: 999-DDY
Plate: 94971 Edit Cell: B11 Edit Date: 2010-12-10 Status: Success Previous Location: Plate: 94971 Cell: B11
Plate: 94972 Edit Cell: C12 Edit Date: 2010-12-14 Status: Success Previous Location: Plate: 94972 Cell: C12

STAGE: Plasmid Purification

Plate: 108955 Edit (C-1076) Cell: C01 Edit Date: 2012-06-07 Status: Success Previous Location: Plate: 108955 (C-1076) Cell: C01

Clone ID: 108348 Date: 2012-06-08
Strain/Vector: PB1 Date: 2012-06-08
Clone Site: SpeedET Date: 2012-06-08
Cloned Date: 2012-06-07 Date: 2012-07-07

Plate: 25095 Edit (C-9999) Cell: 999-APO Edit Date: 2010-12-18 Status: Success Previous Location: Plate: 25095 (C-9999) Cell: 999-APO

Clone ID: 85982 Date: 2010-12-18
Strain/Vector: HEK293 Date: 2010-12-18
Clone Site: pNTC7485speedEhaloT Date: 2010-12-18

Plate: 25095 Edit (C-9999) Cell: 999-DDY Edit Date: 2013-04-09 Status: Success Previous Location: Plate: 25095 (C-9999) Cell: 999-DDY

Clone ID: 125797 Date: 2013-04-09
Strain/Vector: BL21(DE3) Date: 2013-04-09
Clone Site: SpeedE6T Date: 2013-04-09

Plate: 94971 Edit (C-0917) Cell: B11 Edit Date: 2010-12-10 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Clone ID: 85779 Date: 2010-12-18
Strain/Vector: HK100 Date: 2010-12-18
Clone Site: SpeedET Date: 2010-12-18
Cloned Date: 2010-12-10 Date: 2011-03-24

Plate: 94972 Edit (C-0918) Cell: C12 Edit Date: 2010-12-14 Status: Success Previous Location: Plate: 94972 (C-0918) Cell: C12

Clone ID: 85887 Date: 2010-12-18
Strain/Vector: HK100 Date: 2010-12-18
Clone Site: pNTC7485speedEhaloT Date: 2010-12-18
Cloned Date: 2010-12-14 Date: 2011-03-24

STAGE: Expression

Plate: 109001 Edit (E-2517) Cell: C01 Edit Date: 2012-06-08 Status: Success Previous Location: Plate: 108955 (C-1076) Cell: C01

Fermentation Date: 2012-06-08 Date: 2012-06-14
Protein ID: 369654 Date: 2012-06-09
Media: Native-TB Date: 2012-06-09

Plate: 116030 Edit Cell: NMR Edit Date: 2011-12-01 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Protein ID: NMRPID_421651 Date: 2013-04-08

Plate: 127645 Edit (E-2841) Cell: D01 Edit Date: 2016-03-17 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2016-03-17 Date: 2016-04-02
Protein ID: 431814 Date: 2016-04-02
Media: Native-TB Date: 2016-04-02

Plate: 25158 Edit (Expression Attempt) Cell: 999-NL Edit Date: 2012-11-21 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Comment: RNA-12b:GGAGGGUGUGGG, Buffer:20mM Tris-HCl, pH 7.0, 50mM NaCl, 1 mM DTT Date: 2012-11-21
Fermentation Date: 2015-06-17 Date: 2016-04-03
Protein ID: 379060 Date: 2012-11-21
Media: Native-TB Date: 2012-11-21

Plate: 25158 Edit (Expression Attempt) Cell: 999-OT Edit Date: 2013-04-09 Status: Success Previous Location: Plate: 25095 (C-9999) Cell: 999-DDY

Comment: cleaved, RNA-6b:AGGGUA Date: 2013-04-09
Fermentation Date: 2015-06-17 Date: 2016-04-03
Protein ID: 387766 Date: 2013-04-09
Media: Native-TB Date: 2013-04-09

Plate: 25158 Edit (Expression Attempt) Cell: 999-OU Edit Date: 2013-04-09 Status: Success Previous Location: Plate: 25095 (C-9999) Cell: 999-DDY

Comment: cleaved, RNA-6b:AGGGUG Date: 2013-04-09
Fermentation Date: 2015-06-17 Date: 2016-04-03
Protein ID: 387767 Date: 2013-04-09
Media: Native-TB Date: 2013-04-09

Plate: 25158 Edit (Expression Attempt) Cell: 999-PU Edit Date: 2013-07-30 Status: Success Previous Location: Plate: 25095 (C-9999) Cell: 999-DDY

Comment: Complex with RNA 6b:AGGGAU Date: 2013-07-30
Fermentation Date: 2015-06-17 Date: 2016-04-03
Protein ID: 399455 Date: 2013-07-30
Media: Native-TB Date: 2013-07-30

Plate: 95009 Edit (E-2212) Cell: A07 Edit Date: 2011-01-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-01-11 Date: 2012-02-26
GNFuge Date: 2011-01-17 Date: 2012-02-26
Protein ID: 322288 Date: 2011-01-14
Media: Met-Inhibition Date: 2011-01-14

Plate: 95009 Edit (E-2212) Cell: B07 Edit Date: 2011-01-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-01-11 Date: 2012-02-26
GNFuge Date: 2011-01-17 Date: 2012-02-26
Protein ID: 322300 Date: 2011-01-14
Media: Met-Inhibition Date: 2011-01-14

Plate: 95009 Edit (E-2212) Cell: C07 Edit Date: 2011-01-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-01-11 Date: 2012-02-26
GNFuge Date: 2011-01-17 Date: 2012-02-26
Protein ID: 322312 Date: 2011-01-14
Media: Met-Inhibition Date: 2011-01-14

Plate: 95009 Edit (E-2212) Cell: D07 Edit Date: 2011-01-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-01-11 Date: 2012-02-26
GNFuge Date: 2011-01-17 Date: 2012-02-26
Protein ID: 322324 Date: 2011-01-14
Media: Met-Inhibition Date: 2011-01-14

Plate: 95009 Edit (E-2212) Cell: E07 Edit Date: 2011-01-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-01-11 Date: 2012-02-26
GNFuge Date: 2011-01-17 Date: 2012-02-26
Protein ID: 322336 Date: 2011-01-14
Media: Met-Inhibition Date: 2011-01-14

Plate: 95009 Edit (E-2212) Cell: F07 Edit Date: 2011-01-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-01-11 Date: 2012-02-26
GNFuge Date: 2011-01-17 Date: 2012-02-26
Protein ID: 322348 Date: 2011-01-14
Media: Met-Inhibition Date: 2011-01-14

Plate: 95009 Edit (E-2212) Cell: G07 Edit Date: 2011-01-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-01-11 Date: 2012-02-26
GNFuge Date: 2011-01-17 Date: 2012-02-26
Protein ID: 322360 Date: 2011-01-14
Media: Met-Inhibition Date: 2011-01-14

Plate: 95009 Edit (E-2212) Cell: H07 Edit Date: 2011-01-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-01-11 Date: 2012-02-26
GNFuge Date: 2011-01-17 Date: 2012-02-26
Protein ID: 322372 Date: 2011-01-14
Media: Met-Inhibition Date: 2011-01-14

Plate: 95286 Edit (P-0325) Cell: H07 Edit Date: 2011-02-03 Status: Success Previous Location: Plate: 25095 (C-9999) Cell: 999-APO

Fermentation Date: 2011-02-03 Date: 2012-04-22
Protein ID: 324390 Date: 2011-02-05
Media: Freestyle Date: 2011-02-05

Plate: 97179 Edit (E-2292) Cell: A07 Edit Date: 2011-05-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-05-11 Date: 2011-08-21
GNFuge Date: 2011-05-25 Date: 2011-08-21
Protein ID: 334362 Date: 2011-05-13
Media: Met-Inhibition Date: 2011-05-13

Plate: 97179 Edit (E-2292) Cell: B07 Edit Date: 2011-05-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-05-11 Date: 2011-08-21
GNFuge Date: 2011-05-25 Date: 2011-08-21
Protein ID: 334374 Date: 2011-05-13
Media: Met-Inhibition Date: 2011-05-13

Plate: 97179 Edit (E-2292) Cell: C07 Edit Date: 2011-05-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-05-11 Date: 2011-08-21
GNFuge Date: 2011-05-25 Date: 2011-08-21
Protein ID: 334386 Date: 2011-05-13
Media: Met-Inhibition Date: 2011-05-13

Plate: 97179 Edit (E-2292) Cell: D07 Edit Date: 2011-05-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-05-11 Date: 2011-08-21
GNFuge Date: 2011-05-25 Date: 2011-08-21
Protein ID: 334398 Date: 2011-05-13
Media: Met-Inhibition Date: 2011-05-13

Plate: 97179 Edit (E-2292) Cell: E07 Edit Date: 2011-05-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-05-11 Date: 2011-08-21
GNFuge Date: 2011-05-25 Date: 2011-08-21
Protein ID: 334410 Date: 2011-05-13
Media: Met-Inhibition Date: 2011-05-13

Plate: 97179 Edit (E-2292) Cell: F07 Edit Date: 2011-05-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-05-11 Date: 2011-08-21
GNFuge Date: 2011-05-25 Date: 2011-08-21
Protein ID: 334422 Date: 2011-05-13
Media: Met-Inhibition Date: 2011-05-13

Plate: 97179 Edit (E-2292) Cell: G07 Edit Date: 2011-05-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-05-11 Date: 2011-08-21
GNFuge Date: 2011-05-25 Date: 2011-08-21
Protein ID: 334434 Date: 2011-05-13
Media: Met-Inhibition Date: 2011-05-13

Plate: 97179 Edit (E-2292) Cell: H07 Edit Date: 2011-05-11 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-05-11 Date: 2011-08-21
GNFuge Date: 2011-05-25 Date: 2011-08-21
Protein ID: 334446 Date: 2011-05-13
Media: Met-Inhibition Date: 2011-05-13

Plate: 97973 Edit (E-2311) Cell: A11 Edit Date: 2011-06-09 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-06-09 Date: 2011-12-12
Protein ID: 337016 Date: 2011-06-11
Media: Met-Inhibition Date: 2011-06-11

Plate: 97973 Edit (E-2311) Cell: B11 Edit Date: 2011-06-09 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-06-09 Date: 2011-12-12
Protein ID: 337028 Date: 2011-06-11
Media: Met-Inhibition Date: 2011-06-11

Plate: 97973 Edit (E-2311) Cell: C11 Edit Date: 2011-06-09 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-06-09 Date: 2011-12-12
Protein ID: 337040 Date: 2011-06-11
Media: Met-Inhibition Date: 2011-06-11

Plate: 97973 Edit (E-2311) Cell: D11 Edit Date: 2011-06-09 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-06-09 Date: 2011-12-12
Protein ID: 337052 Date: 2011-06-11
Media: Met-Inhibition Date: 2011-06-11

Plate: 97973 Edit (E-2311) Cell: E11 Edit Date: 2011-06-09 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-06-09 Date: 2011-12-12
Protein ID: 337064 Date: 2011-06-11
Media: Met-Inhibition Date: 2011-06-11

Plate: 97973 Edit (E-2311) Cell: F11 Edit Date: 2011-06-09 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-06-09 Date: 2011-12-12
Protein ID: 337076 Date: 2011-06-11
Media: Met-Inhibition Date: 2011-06-11

Plate: 97973 Edit (E-2311) Cell: G11 Edit Date: 2011-06-09 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-06-09 Date: 2011-12-12
Protein ID: 337088 Date: 2011-06-11
Media: Met-Inhibition Date: 2011-06-11

Plate: 97973 Edit (E-2311) Cell: H11 Edit Date: 2011-06-09 Status: Success Previous Location: Plate: 94971 (C-0917) Cell: B11

Fermentation Date: 2011-06-09 Date: 2011-12-12
Protein ID: 337100 Date: 2011-06-11
Media: Met-Inhibition Date: 2011-06-11

STAGE: Protein Purification

Plate: 109001 Edit (E-2517) Cell: C01 Edit Date: 2012-06-09 Status: Success Previous Location: Plate: 109001 (E-2517) Cell: C01
Plate: 116127 Edit Cell: NMR Edit Date: 2011-12-01 Status: Success Previous Location: Plate: 116030 Cell: NMR
Plate: 127645 Edit (E-2841) Cell: D01 Edit Date: 2016-04-02 Status: Success Previous Location: Plate: 127645 (E-2841) Cell: D01
Plate: 25158 Edit (Expression Attempt) Cell: 999-NL Edit Date: 2012-11-21 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL

Purified Concentration (mg/mL): 10.0 Date: 2012-11-21

Plate: 25158 Edit (Expression Attempt) Cell: 999-OT Edit Date: 2013-04-09 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT
Plate: 25158 Edit (Expression Attempt) Cell: 999-OU Edit Date: 2013-04-09 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU
Plate: 25158 Edit (Expression Attempt) Cell: 999-PU Edit Date: 2013-07-30 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-PU
Plate: 95009 Edit (E-2212) Cell: A07 Edit Date: 2011-01-14 Status: Success Previous Location: Plate: 95009 (E-2212) Cell: A07

Purified Concentration (mg/mL): 20.0 Date: 2011-01-27

Plate: 95009 Edit (E-2212) Cell: B07 Edit Date: 2011-01-14 Status: Success Previous Location: Plate: 95009 (E-2212) Cell: B07
Plate: 95009 Edit (E-2212) Cell: C07 Edit Date: 2011-01-14 Status: Success Previous Location: Plate: 95009 (E-2212) Cell: C07
Plate: 95009 Edit (E-2212) Cell: D07 Edit Date: 2011-01-14 Status: Success Previous Location: Plate: 95009 (E-2212) Cell: D07
Plate: 95009 Edit (E-2212) Cell: E07 Edit Date: 2011-01-14 Status: Success Previous Location: Plate: 95009 (E-2212) Cell: E07
Plate: 95009 Edit (E-2212) Cell: F07 Edit Date: 2011-01-14 Status: Success Previous Location: Plate: 95009 (E-2212) Cell: F07
Plate: 95009 Edit (E-2212) Cell: G07 Edit Date: 2011-01-14 Status: Success Previous Location: Plate: 95009 (E-2212) Cell: G07
Plate: 95009 Edit (E-2212) Cell: H07 Edit Date: 2011-01-14 Status: Success Previous Location: Plate: 95009 (E-2212) Cell: H07
Plate: 95286 Edit (P-0325) Cell: H07 Edit Date: 2011-02-05 Status: Success Previous Location: Plate: 95286 (P-0325) Cell: H07
Plate: 97179 Edit (E-2292) Cell: A07 Edit Date: 2011-05-13 Status: Success Previous Location: Plate: 97179 (E-2292) Cell: A07

Purified Concentration (mg/mL): 14.2 Date: 2011-06-05

Plate: 97179 Edit (E-2292) Cell: B07 Edit Date: 2011-05-13 Status: Success Previous Location: Plate: 97179 (E-2292) Cell: B07
Plate: 97179 Edit (E-2292) Cell: C07 Edit Date: 2011-05-13 Status: Success Previous Location: Plate: 97179 (E-2292) Cell: C07
Plate: 97179 Edit (E-2292) Cell: D07 Edit Date: 2011-05-13 Status: Success Previous Location: Plate: 97179 (E-2292) Cell: D07
Plate: 97179 Edit (E-2292) Cell: E07 Edit Date: 2011-05-13 Status: Success Previous Location: Plate: 97179 (E-2292) Cell: E07
Plate: 97179 Edit (E-2292) Cell: F07 Edit Date: 2011-05-13 Status: Success Previous Location: Plate: 97179 (E-2292) Cell: F07
Plate: 97179 Edit (E-2292) Cell: G07 Edit Date: 2011-05-13 Status: Success Previous Location: Plate: 97179 (E-2292) Cell: G07
Plate: 97179 Edit (E-2292) Cell: H07 Edit Date: 2011-05-13 Status: Success Previous Location: Plate: 97179 (E-2292) Cell: H07
Plate: 97973 Edit (E-2311) Cell: A11 Edit Date: 2011-06-11 Status: Success Previous Location: Plate: 97973 (E-2311) Cell: A11
Plate: 97973 Edit (E-2311) Cell: B11 Edit Date: 2011-06-11 Status: Success Previous Location: Plate: 97973 (E-2311) Cell: B11
Plate: 97973 Edit (E-2311) Cell: C11 Edit Date: 2011-06-11 Status: Success Previous Location: Plate: 97973 (E-2311) Cell: C11
Plate: 97973 Edit (E-2311) Cell: D11 Edit Date: 2011-06-11 Status: Success Previous Location: Plate: 97973 (E-2311) Cell: D11
Plate: 97973 Edit (E-2311) Cell: E11 Edit Date: 2011-06-11 Status: Success Previous Location: Plate: 97973 (E-2311) Cell: E11
Plate: 97973 Edit (E-2311) Cell: F11 Edit Date: 2011-06-11 Status: Success Previous Location: Plate: 97973 (E-2311) Cell: F11
Plate: 97973 Edit (E-2311) Cell: G11 Edit Date: 2011-06-11 Status: Success Previous Location: Plate: 97973 (E-2311) Cell: G11
Plate: 97973 Edit (E-2311) Cell: H11 Edit Date: 2011-06-11 Status: Success Previous Location: Plate: 97973 (E-2311) Cell: H11

STAGE: HSQC satisfactory

NMR Tube: 116239 Edit Cell: NMR Edit Date: 2011-12-01 Status: In Progress Previous Location: Plate: 116127 Cell: NMR

STAGE: [13C,15N] - labeled sample

NMR Tube: 116280 Edit Cell: NMR Edit Date: 2011-12-01 Status: In Progress Previous Location: NMR Tube: 116239 Cell: NMR

STAGE: Crystallization

Plate: 112901 Edit (UZ004652) Cell: XT Edit Date: 2012-11-21 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL

Screen Type: Sparse Matrix Date: 2012-12-30
Setup Date: 2012-11-21 Date: 2012-11-22
Setup Temp (C): 4 Date: 2012-11-22
Crystal Conditions: The JCSG Core Suite I Date: 2012-12-30
Drop Volume (nl): 200 Date: 2012-12-30
Reservoir Volume (ul): 35 Date: 2012-12-30
CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30

Plate: 112904 Edit (UZ004655) Cell: XT Edit Date: 2012-11-21 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL

Screen Type: Sparse Matrix Date: 2012-12-30
Setup Date: 2012-11-21 Date: 2012-11-22
Setup Temp (C): 4 Date: 2012-11-22
Crystal Conditions: The JCSG Core Suite II Date: 2012-12-30
Drop Volume (nl): 200 Date: 2012-12-30
Reservoir Volume (ul): 35 Date: 2012-12-30
CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30

Plate: 112907 Edit (UZ004658) Cell: XT Edit Date: 2012-11-21 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL

Screen Type: Sparse Matrix Date: 2012-12-30
Setup Date: 2012-11-21 Date: 2012-11-22
Setup Temp (C): 4 Date: 2012-11-22
Crystal Conditions: The JCSG Core Suite III Date: 2012-12-30
Drop Volume (nl): 200 Date: 2012-12-30
Reservoir Volume (ul): 35 Date: 2012-12-30
CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30

Plate: 112910 Edit (UZ004661) Cell: XT Edit Date: 2012-11-21 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL

Screen Type: Sparse Matrix Date: 2012-12-30
Setup Date: 2012-11-21 Date: 2012-11-22
Setup Temp (C): 4 Date: 2012-11-22
Crystal Conditions: The JCSG Core Suite IV Date: 2012-12-30
Drop Volume (nl): 200 Date: 2012-12-30
Reservoir Volume (ul): 35 Date: 2012-12-30
CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30

Plate: 113069 Edit (S0035613) Cell: XT Edit Date: 2012-11-29 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL

Screen Type: Sparse Matrix Date: 2012-12-30
Setup Date: 2012-11-29 Date: 2012-11-30
Setup Temp (C): 4 Date: 2012-11-30
Crystal Conditions: The Nucleix Suite Date: 2012-12-30
Drop Volume (nl): 200 Date: 2012-12-30
Reservoir Volume (ul): 50 Date: 2012-12-30
CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30

Plate: 113070 Edit (S0035615) Cell: XT Edit Date: 2012-11-29 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-NL

Screen Type: Sparse Matrix Date: 2012-12-30
Setup Date: 2012-11-29 Date: 2012-11-30
Setup Temp (C): 4 Date: 2012-11-30
Crystal Conditions: The Protein Complex Suite Date: 2012-12-30
Drop Volume (nl): 200 Date: 2012-12-30
Reservoir Volume (ul): 50 Date: 2012-12-30
CoCrystal Compound: 1mM 12mer RNA: GGAGGGUGUGGG Date: 2012-12-30

Plate: 116426 Edit (UZ007080) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 20 Date: 2013-04-14
Crystal Conditions: The Protein Complex Suite Date: 2014-05-22
Drop Volume (nl): 200 Date: 2014-05-22
Reservoir Volume (ul): 35 Date: 2014-05-22
CoCrystal Compound: 1mM RNA-6b:AGGGUA Date: 2014-05-22

Plate: 116427 Edit (UZ007082) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 4 Date: 2013-04-14
Crystal Conditions: GNF1 Date: 2014-05-30
Drop Volume (nl): 100.0 Date: 2014-05-30
Precipitate Volume (nl): 100 Date: 2014-05-30
Reservoir Volume (ul): 50.0 Date: 2014-05-30
CoCrystal Compound: RNA-6b:AGGGUA 1 mM Date: 2014-05-30

Plate: 116428 Edit (UZ007083) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 20 Date: 2013-04-14
Crystal Conditions: GNF1 Date: 2014-05-30
Drop Volume (nl): 100.0 Date: 2014-05-30
Precipitate Volume (nl): 100 Date: 2014-05-30
Reservoir Volume (ul): 50.0 Date: 2014-05-30
CoCrystal Compound: RNA-6b:AGGGUA 1 mM Date: 2014-05-30

Plate: 116429 Edit (UZ007084) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 4 Date: 2013-04-14
Crystal Conditions: Top96 Date: 2013-04-14
Drop Volume (nl): 200 Date: 2014-05-22
Reservoir Volume (ul): 35 Date: 2014-05-22
CoCrystal Compound: 1mM RNA-6b:AGGGUG Date: 2014-05-22

Plate: 116430 Edit (UZ007085) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 20 Date: 2013-04-14
Crystal Conditions: Top96 Date: 2013-04-14
Drop Volume (nl): 200 Date: 2014-05-22
Reservoir Volume (ul): 35 Date: 2014-05-22
CoCrystal Compound: 1mM RNA-6b:AGGGUG Date: 2014-05-22

Plate: 116431 Edit (UZ007086) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 4 Date: 2013-04-14
Crystal Conditions: Top96 Date: 2013-04-14
Drop Volume (nl): 200 Date: 2014-05-22
Reservoir Volume (ul): 35 Date: 2014-05-22
CoCrystal Compound: 1mM RNA-6b:AGGGUA Date: 2014-05-22

Plate: 116432 Edit (UZ007087) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 20 Date: 2013-04-14
Crystal Conditions: Top96 Date: 2013-04-14
Drop Volume (nl): 200 Date: 2014-05-22
Reservoir Volume (ul): 35 Date: 2014-05-22
CoCrystal Compound: 1mM RNA-6b:AGGGUA Date: 2014-05-22

Plate: 116433 Edit (UZ007089) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT

Screen Type: Fine Date: 2013-04-14
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 20 Date: 2013-04-14
Crystal Conditions: UZ007089-NatrixHT Date: 2014-05-24
Drop Volume (nl): 100.0 Date: 2014-05-24
Precipitate Volume (nl): 100 Date: 2014-05-24
Reservoir Volume (ul): 50.0 Date: 2014-05-24
CoCrystal Compound: RNA-6b:AGGGUA 1 mM Date: 2014-05-24

Plate: 116434 Edit (UZ007090) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 4 Date: 2013-04-14
Crystal Conditions: The Protein Complex Suite Date: 2014-05-22
Drop Volume (nl): 200 Date: 2014-05-22
Reservoir Volume (ul): 35 Date: 2014-05-22
CoCrystal Compound: 1mM RNA-6b:AGGGUG Date: 2014-05-22

Plate: 116435 Edit (UZ007091) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 20 Date: 2013-04-14
Crystal Conditions: The Protein Complex Suite Date: 2014-05-22
Drop Volume (nl): 200 Date: 2014-05-22
Reservoir Volume (ul): 35 Date: 2014-05-22
CoCrystal Compound: 1mM RNA-6b:AGGGUG Date: 2014-05-22

Plate: 116436 Edit (UZ007092) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 4 Date: 2013-04-14
Crystal Conditions: The Protein Complex Suite Date: 2014-05-22
Drop Volume (nl): 200 Date: 2014-05-22
Reservoir Volume (ul): 35 Date: 2014-05-22
CoCrystal Compound: 1mM RNA-6b:AGGGUA Date: 2014-05-22

Plate: 116437 Edit (UZ007093) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 4 Date: 2013-04-14
Crystal Conditions: GNF1 Date: 2014-05-30
Drop Volume (nl): 100.0 Date: 2014-05-30
Precipitate Volume (nl): 100 Date: 2014-05-30
Reservoir Volume (ul): 50.0 Date: 2014-05-30
CoCrystal Compound: RNA-6b:AGGGUG 1 mM Date: 2014-05-30

Plate: 116438 Edit (UZ007094) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU

Screen Type: Sparse Matrix Date: 2014-05-22
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 20 Date: 2013-04-14
Crystal Conditions: GNF1 Date: 2014-05-30
Drop Volume (nl): 100.0 Date: 2014-05-30
Precipitate Volume (nl): 100 Date: 2014-05-30
Reservoir Volume (ul): 50.0 Date: 2014-05-30
CoCrystal Compound: RNA-6b:AGGGUG 1 mM Date: 2014-05-30

Plate: 116439 Edit (UZ007096) Cell: XT Edit Date: 2013-04-13 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OT

Screen Type: Fine Date: 2013-04-14
Setup Date: 2013-04-13 Date: 2013-04-14
Setup Temp (C): 4 Date: 2013-04-14
Crystal Conditions: UZ007096-NatrixHT Date: 2014-05-24
Drop Volume (nl): 100.0 Date: 2014-05-24
Precipitate Volume (nl): 100 Date: 2014-05-24
Reservoir Volume (ul): 50.0 Date: 2014-05-24
CoCrystal Compound: RNA-6b:AGGGUA 1 mM Date: 2014-05-24

Plate: 116455 Edit (UZ007148) Cell: XT Edit Date: 2013-04-15 Status: Success Previous Location: Plate: 25158 (Expression Attempt) Cell: 999-OU

Screen Type: Fine Date: 2013-04-16
Setup Date: 2013-04-15 Date: 2013-04-16
Setup Temp (C): 20 Date: 2013-04-16
Crystal Conditions: UZ007148-NatrixHT Date: 2014-05-24
Drop Volume (nl): 100.0 Date: 2014-05-24
Precipitate Volume (nl): 100 Date: 2014-05-24
Reservoir Volume (ul): 50.0 Date: 2014-05-24
CoCrystal Compound: RNA-6b:AGGGUG 1 mM Date: 2014-05-24

Plate: 95390 Edit</