JCSG Target History

Target ID:	375102
Target Name:	HP10638A		Protein Accession:	YP_676701.1		Local Accession:	HP10638A		cDNA Accession:	REF\|NC_008255.1\|:80866-81774		PSCA	PDB:3cpx

Target ID:

375102

Target Name:

HP10638A

Protein Accession:

YP_676701.1

Local Accession:

HP10638A

cDNA Accession:

REF|NC_008255.1|:80866-81774

PSCA

PDB:3cpx

Hover over nodes to see experimental data. Click on nodes to go to that point in the data below.

Proposed Target

Active Target

PCR Testing

Amplifer Generation

Orientation

Plasmid Purification

Micro Expression

Expression

Protein Purification

Crystallization

Crystal Hit

SDC Receiving

Screening

Data Collection

Integration

Scaling

Truncating

Phasing

Density Modification

Tracing

Refining

Validation

PDB Deposition

Proposed Target

Active Target

PCR Testing

Amplifer Generation

Orientation

Plasmid Purification

Micro Expression

Expression

Protein Purification

Crystallization

Crystal Hit

SDC Receiving

Screening

Data Collection

Integration

Scaling

Truncating

Phasing

Density Modification

Tracing

Refining

Validation

PDB Deposition

Status View


Chrono View

Name:	putative M42 glutamyl aminopeptidase	Start Position:	1	End Position:	302
GNF Target Alias:	HK100:SpeedET-HP10638A-chu-302-1-302	Sequence Type:	COMPLETE	Residues:	302
DNA Length:	906	Molecular Weight:	34277.17	Isoelectric Point:	4.90
Epsilon 280:	61880	Gravy Index:	-0.26	Species:	C. hutchinsonii ATCC 33406
Activated:	1	Alanine Absolute:	18	Alanine Relative:	0.0596
Arginine Absolute:	14	Arginine Relative:	0.0464	Asparagine Absolute:	9
Asparagine Relative:	0.0298	Aspartic Acid Absolute:	21	Aspartic Acid Relative:	0.0695
Cysteine Absolute:	6	Cysteine Relative:	0.0199	Glutamic Acid Absolute:	27
Glutamic Acid Relative:	0.0894	Glutamine Absolute:	7	Glutamine Relative:	0.0232
Glycine Absolute:	24	Glycine Relative:	0.0795	Histidine Absolute:	9
Histidine Relative:	0.0298	Isoleucine Absolute:	23	Isoleucine Relative:	0.0762
Leucine Absolute:	27	Leucine Relative:	0.0894	Lysine Absolute:	16
Lysine Relative:	0.0530	Methionine Absolute:	7	Methionine Relative:	0.0232
Phenylalanine Absolute:	11	Phenylalanine Relative:	0.0364	Proline Absolute:	14
Proline Relative:	0.0464	Serine Absolute:	16	Serine Relative:	0.0530
Threonine Absolute:	14	Threonine Relative:	0.0464	Tryptophan Absolute:	8
Tryptophan Relative:	0.0265	Tyrosine Absolute:	12	Tyrosine Relative:	0.0397
Valine Absolute:	19	Valine Relative:	0.0629	Project:	Zn peptidases
Selection Date:	2007-02-27

STAGE: Proposed Target

Plate: 0 Cell: 375102 Date: 2007-02-27 Status: Success

STAGE: Active Target

Plate: 45932 Cell: H04 Date: 2007-03-22 Status: Success Previous Location: Plate: 0 Cell: 375102

Sample Name: HK100:SpeedET-HP10638A-chu-302-1-302 Date: 2008-10-19
GNF Project Name: PSI2_LargePFAMproj5_230(FB-HP-RK) Date: 2008-10-19
GNF Program Name: JCSG Date: 2008-10-19

STAGE: PCR Testing

Plate: 45932 Cell: H04 Date: 2007-03-22 Status: Success Previous Location: Plate: 45932 Cell: H04

STAGE: Amplifer Generation

Plate: 45932 Cell: H04 Date: 2007-03-22 Status: Success Previous Location: Plate: 45932 Cell: H04

STAGE: Orientation

Plate: 45932 Cell: H04 Date: 2007-03-22 Status: Success Previous Location: Plate: 45932 Cell: H04

STAGE: Plasmid Purification

Plate: 45932 (C-0475) Cell: H04 Date: 2007-03-22 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Clone ID: 32169 Date: 2007-03-24
Strain/Vector: HK100 Date: 2008-10-19
Clone Site: SpeedET Date: 2008-10-19
Cloned Date: 2007-03-22 Date: 2008-10-19

STAGE: Micro Expression

Plate: 45992 Cell: H04 Date: 2007-03-23 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04
Plate: 59469 Cell: C07 Date: 2008-02-12 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04
Plate: 60392 Cell: C07 Date: 2008-03-12 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

STAGE: Expression

Plate: 47552 (E-1115) Cell: A05 Date: 2007-04-25 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Comment: His-tag's on Date: 2008-10-19
Fermentation Date: 2007-04-25 Date: 2008-10-19
GNFuge Date: 2007-11-29 Date: 2008-10-19
Protein ID: 99513 Date: 2007-05-08
Media: Met-Inhibition Date: 2008-10-19

Plate: 47552 (E-1115) Cell: B05 Date: 2007-04-25 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2007-04-25 Date: 2008-10-19
GNFuge Date: 2007-11-29 Date: 2008-10-19
Protein ID: 99525 Date: 2007-05-08
Media: Met-Inhibition Date: 2008-10-19

Plate: 47552 (E-1115) Cell: C05 Date: 2007-04-25 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2007-04-25 Date: 2008-10-19
GNFuge Date: 2007-11-29 Date: 2008-10-19
Protein ID: 99537 Date: 2007-05-08
Media: Met-Inhibition Date: 2008-10-19

Plate: 47552 (E-1115) Cell: D05 Date: 2007-04-25 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2007-04-25 Date: 2008-10-19
GNFuge Date: 2007-11-29 Date: 2008-10-19
Protein ID: 99549 Date: 2007-05-08
Media: Met-Inhibition Date: 2008-10-19

Plate: 47552 (E-1115) Cell: E05 Date: 2007-04-25 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2007-04-25 Date: 2008-10-19
GNFuge Date: 2007-11-29 Date: 2008-10-19
Protein ID: 99561 Date: 2007-05-08
Media: Met-Inhibition Date: 2008-10-19

Plate: 47552 (E-1115) Cell: F05 Date: 2007-04-25 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2007-04-25 Date: 2008-10-19
GNFuge Date: 2007-11-29 Date: 2008-10-19
Protein ID: 99573 Date: 2007-05-08
Media: Met-Inhibition Date: 2008-10-19

Plate: 47552 (E-1115) Cell: G05 Date: 2007-04-25 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2007-04-25 Date: 2008-10-19
GNFuge Date: 2007-11-29 Date: 2008-10-19
Protein ID: 99585 Date: 2007-05-08
Media: Met-Inhibition Date: 2008-10-19

Plate: 47552 (E-1115) Cell: H05 Date: 2007-04-25 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2007-04-25 Date: 2008-10-19
GNFuge Date: 2007-11-29 Date: 2008-10-19
Protein ID: 99597 Date: 2007-05-08
Media: Native-TB Date: 2008-10-19

Plate: 62193 (E-1442) Cell: A08 Date: 2008-05-15 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Comment: His-tag's on Date: 2008-10-19
Fermentation Date: 2008-05-15 Date: 2008-10-19
Protein ID: 134398 Date: 2008-05-17
Media: Met-Inhibition Date: 2008-10-19

Plate: 62193 (E-1442) Cell: B08 Date: 2008-05-15 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-05-15 Date: 2008-10-19
Protein ID: 134410 Date: 2008-05-17
Media: Met-Inhibition Date: 2008-10-19

Plate: 62193 (E-1442) Cell: C08 Date: 2008-05-15 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-05-15 Date: 2008-10-19
Protein ID: 134422 Date: 2008-05-17
Media: Met-Inhibition Date: 2008-10-19

Plate: 62193 (E-1442) Cell: D08 Date: 2008-05-15 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-05-15 Date: 2008-10-19
Protein ID: 134434 Date: 2008-05-17
Media: Met-Inhibition Date: 2008-10-19

Plate: 62193 (E-1442) Cell: E08 Date: 2008-05-15 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-05-15 Date: 2008-10-19
Protein ID: 134446 Date: 2008-05-17
Media: Met-Inhibition Date: 2008-10-19

Plate: 62193 (E-1442) Cell: F08 Date: 2008-05-15 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-05-15 Date: 2008-10-19
Protein ID: 134458 Date: 2008-05-17
Media: Met-Inhibition Date: 2008-10-19

Plate: 62193 (E-1442) Cell: G08 Date: 2008-05-15 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-05-15 Date: 2008-10-19
Protein ID: 134470 Date: 2008-05-17
Media: Met-Inhibition Date: 2008-10-19

Plate: 62193 (E-1442) Cell: H08 Date: 2008-05-15 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-05-15 Date: 2008-10-19
Protein ID: 134482 Date: 2008-05-17
Media: Met-Inhibition Date: 2008-10-19

Plate: 64591 (E-1507) Cell: F05 Date: 2008-07-08 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-07-08 Date: 2010-07-14
Protein ID: 141470 Date: 2008-07-10
Media: Native-TB Date: 2008-10-19

Plate: 64591 (E-1507) Cell: F06 Date: 2008-07-08 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-07-08 Date: 2010-07-14
Protein ID: 141471 Date: 2008-07-10
Media: Native-TB Date: 2008-10-19

Plate: 66815 (E-1575) Cell: A01 Date: 2008-08-27 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-08-28 Date: 2009-06-18
GNFuge Date: 2008-11-11 Date: 2009-06-18
Protein ID: 148953 Date: 2008-08-27
Media: Met-Inhibition Date: 2008-10-19

Plate: 66815 (E-1575) Cell: B01 Date: 2008-08-27 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-08-28 Date: 2009-06-18
GNFuge Date: 2008-11-11 Date: 2009-06-18
Protein ID: 148965 Date: 2008-08-27
Media: Met-Inhibition Date: 2008-10-19

Plate: 66815 (E-1575) Cell: C01 Date: 2008-08-27 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-08-28 Date: 2009-06-18
GNFuge Date: 2008-11-11 Date: 2009-06-18
Protein ID: 148977 Date: 2008-08-27
Media: Met-Inhibition Date: 2008-10-19

Plate: 66815 (E-1575) Cell: D01 Date: 2008-08-27 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-08-28 Date: 2009-06-18
GNFuge Date: 2008-11-11 Date: 2009-06-18
Protein ID: 148989 Date: 2008-08-27
Media: Met-Inhibition Date: 2008-10-19

Plate: 66815 (E-1575) Cell: E01 Date: 2008-08-27 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-08-28 Date: 2009-06-18
GNFuge Date: 2008-11-11 Date: 2009-06-18
Protein ID: 149001 Date: 2008-08-27
Media: Met-Inhibition Date: 2008-10-19

Plate: 66815 (E-1575) Cell: F01 Date: 2008-08-27 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-08-28 Date: 2009-06-18
GNFuge Date: 2008-11-11 Date: 2009-06-18
Protein ID: 149013 Date: 2008-08-27
Media: Met-Inhibition Date: 2008-10-19

Plate: 66815 (E-1575) Cell: G01 Date: 2008-08-27 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-08-28 Date: 2009-06-18
GNFuge Date: 2008-11-11 Date: 2009-06-18
Protein ID: 149025 Date: 2008-08-27
Media: Met-Inhibition Date: 2008-10-19

Plate: 66815 (E-1575) Cell: H01 Date: 2008-08-27 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-08-28 Date: 2009-06-18
GNFuge Date: 2008-11-11 Date: 2009-06-18
Protein ID: 149037 Date: 2008-08-27
Media: Met-Inhibition Date: 2008-10-19

Plate: 68284 (E-1590) Cell: A01 Date: 2008-09-17 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-09-17 Date: 2009-06-18
GNFuge Date: 2008-11-17 Date: 2009-06-18
Protein ID: 151285 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-10-25

Plate: 68284 (E-1590) Cell: B01 Date: 2008-09-17 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-09-17 Date: 2009-06-18
GNFuge Date: 2008-11-17 Date: 2009-06-18
Protein ID: 151297 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-10-25

Plate: 68284 (E-1590) Cell: C01 Date: 2008-09-17 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-09-17 Date: 2009-06-18
GNFuge Date: 2008-11-17 Date: 2009-06-18
Protein ID: 151309 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-10-25

Plate: 68284 (E-1590) Cell: D01 Date: 2008-09-17 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-09-17 Date: 2009-06-18
GNFuge Date: 2008-11-17 Date: 2009-06-18
Protein ID: 151321 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-10-25

Plate: 68284 (E-1590) Cell: E01 Date: 2008-09-17 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-09-17 Date: 2009-06-18
GNFuge Date: 2008-11-17 Date: 2009-06-18
Protein ID: 151333 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-10-25

Plate: 68284 (E-1590) Cell: F01 Date: 2008-09-17 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-09-17 Date: 2009-06-18
GNFuge Date: 2008-11-17 Date: 2009-06-18
Protein ID: 151345 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-10-25

Plate: 68284 (E-1590) Cell: G01 Date: 2008-09-17 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-09-17 Date: 2009-06-18
GNFuge Date: 2008-11-17 Date: 2009-06-18
Protein ID: 151357 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-10-25

Plate: 68284 (E-1590) Cell: H01 Date: 2008-09-17 Status: Success Previous Location: Plate: 45932 (C-0475) Cell: H04

Fermentation Date: 2008-09-17 Date: 2009-06-18
GNFuge Date: 2008-11-17 Date: 2009-06-18
Protein ID: 151369 Date: 2008-09-26
Media: Met-Inhibition Date: 2008-10-25

STAGE: Protein Purification

Plate: 47552 (E-1115) Cell: A05 Date: 2007-05-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: A05

Purified Concentration (mg/mL): 20.0 Date: 2008-10-19

Plate: 47552 (E-1115) Cell: B05 Date: 2007-05-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: B05
Plate: 47552 (E-1115) Cell: C05 Date: 2007-05-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: C05
Plate: 47552 (E-1115) Cell: D05 Date: 2007-05-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: D05
Plate: 47552 (E-1115) Cell: E05 Date: 2007-05-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: E05
Plate: 47552 (E-1115) Cell: F05 Date: 2007-05-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: F05
Plate: 47552 (E-1115) Cell: G05 Date: 2007-05-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: G05
Plate: 47552 (E-1115) Cell: H05 Date: 2007-05-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: H05
Plate: 62193 (E-1442) Cell: A08 Date: 2008-05-17 Status: Success Previous Location: Plate: 62193 (E-1442) Cell: A08

Purified Concentration (mg/mL): 10.0 Date: 2008-10-19

Plate: 62193 (E-1442) Cell: B08 Date: 2008-05-17 Status: Success Previous Location: Plate: 62193 (E-1442) Cell: B08
Plate: 62193 (E-1442) Cell: C08 Date: 2008-05-17 Status: Success Previous Location: Plate: 62193 (E-1442) Cell: C08
Plate: 62193 (E-1442) Cell: D08 Date: 2008-05-17 Status: Success Previous Location: Plate: 62193 (E-1442) Cell: D08
Plate: 62193 (E-1442) Cell: E08 Date: 2008-05-17 Status: Success Previous Location: Plate: 62193 (E-1442) Cell: E08
Plate: 62193 (E-1442) Cell: F08 Date: 2008-05-17 Status: Success Previous Location: Plate: 62193 (E-1442) Cell: F08
Plate: 62193 (E-1442) Cell: G08 Date: 2008-05-17 Status: Success Previous Location: Plate: 62193 (E-1442) Cell: G08
Plate: 62193 (E-1442) Cell: H08 Date: 2008-05-17 Status: Success Previous Location: Plate: 62193 (E-1442) Cell: H08
Plate: 64591 (E-1507) Cell: F05 Date: 2008-07-10 Status: Success Previous Location: Plate: 64591 (E-1507) Cell: F05
Plate: 64591 (E-1507) Cell: F06 Date: 2008-07-10 Status: Success Previous Location: Plate: 64591 (E-1507) Cell: F06
Plate: 66815 (E-1575) Cell: A01 Date: 2008-08-27 Status: Success Previous Location: Plate: 66815 (E-1575) Cell: A01
Plate: 66815 (E-1575) Cell: B01 Date: 2008-08-27 Status: Success Previous Location: Plate: 66815 (E-1575) Cell: B01
Plate: 66815 (E-1575) Cell: C01 Date: 2008-08-27 Status: Success Previous Location: Plate: 66815 (E-1575) Cell: C01
Plate: 66815 (E-1575) Cell: D01 Date: 2008-08-27 Status: Success Previous Location: Plate: 66815 (E-1575) Cell: D01
Plate: 66815 (E-1575) Cell: E01 Date: 2008-08-27 Status: Success Previous Location: Plate: 66815 (E-1575) Cell: E01
Plate: 66815 (E-1575) Cell: F01 Date: 2008-08-27 Status: Success Previous Location: Plate: 66815 (E-1575) Cell: F01
Plate: 66815 (E-1575) Cell: G01 Date: 2008-08-27 Status: Success Previous Location: Plate: 66815 (E-1575) Cell: G01
Plate: 66815 (E-1575) Cell: H01 Date: 2008-08-27 Status: Success Previous Location: Plate: 66815 (E-1575) Cell: H01
Plate: 68284 (E-1590) Cell: A01 Date: 2008-09-26 Status: Success Previous Location: Plate: 68284 (E-1590) Cell: A01
Plate: 68284 (E-1590) Cell: B01 Date: 2008-09-26 Status: Success Previous Location: Plate: 68284 (E-1590) Cell: B01
Plate: 68284 (E-1590) Cell: C01 Date: 2008-09-26 Status: Success Previous Location: Plate: 68284 (E-1590) Cell: C01
Plate: 68284 (E-1590) Cell: D01 Date: 2008-09-26 Status: Success Previous Location: Plate: 68284 (E-1590) Cell: D01
Plate: 68284 (E-1590) Cell: E01 Date: 2008-09-26 Status: Success Previous Location: Plate: 68284 (E-1590) Cell: E01
Plate: 68284 (E-1590) Cell: F01 Date: 2008-09-26 Status: Success Previous Location: Plate: 68284 (E-1590) Cell: F01
Plate: 68284 (E-1590) Cell: G01 Date: 2008-09-26 Status: Success Previous Location: Plate: 68284 (E-1590) Cell: G01
Plate: 68284 (E-1590) Cell: H01 Date: 2008-09-26 Status: Success Previous Location: Plate: 68284 (E-1590) Cell: H01

STAGE: Crystallization

Plate: 48803 (G0033495) Cell: XT Date: 2007-06-01 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: A05

Screen Type: Sparse Matrix Date: 2008-10-19
Setup Date: 2007-06-01 Date: 2010-11-22
Setup Temp: 4 Date: 2008-10-19
Crystal Conditions: GNF1 Date: 2008-10-19
Drop Volume (nl): 200.0 Date: 2008-10-19
Precipitate Volume (nl): 200 Date: 2008-10-19
Reservoir Volume (ul): 50.0 Date: 2008-10-19

Plate: 48804 (G0033357) Cell: XT Date: 2007-06-01 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: A05

Screen Type: Sparse Matrix Date: 2008-10-19
Setup Date: 2007-06-01 Date: 2008-10-19
Setup Temp: 4 Date: 2008-10-19
Crystal Conditions: GNF2 Date: 2008-10-19
Drop Volume (nl): 200.0 Date: 2008-10-19
Precipitate Volume (nl): 200 Date: 2008-10-19
Reservoir Volume (ul): 50.0 Date: 2008-10-19

Plate: 48805 (G0027640) Cell: XT Date: 2007-06-01 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: A05

Screen Type: Sparse Matrix Date: 2008-10-19
Setup Date: 2007-06-01 Date: 2008-10-19
Setup Temp: 4 Date: 2008-10-19
Crystal Conditions: GNF3 Date: 2008-10-19
Drop Volume (nl): 200.0 Date: 2008-10-19
Precipitate Volume (nl): 200 Date: 2008-10-19
Reservoir Volume (ul): 50.0 Date: 2008-10-19

Plate: 48806 (G0033259) Cell: XT Date: 2007-06-01 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: A05

Screen Type: Sparse Matrix Date: 2008-10-19
Setup Date: 2007-06-01 Date: 2008-10-19
Setup Temp: 4 Date: 2008-10-19
Crystal Conditions: GNF4 Date: 2008-10-19
Drop Volume (nl): 200.0 Date: 2008-10-19
Precipitate Volume (nl): 200 Date: 2008-10-19
Reservoir Volume (ul): 50.0 Date: 2008-10-19

Plate: 53774 (S0003843) Cell: XT Date: 2007-10-02 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: A05

Screen Type: Fine Date: 2008-10-19
Setup Date: 2007-10-02 Date: 2010-10-01
Setup Temp: 4 Date: 2008-10-19
Crystal Conditions: S0003843-GNF2-E04 Date: 2008-10-19
Drop Volume (nl): 100.0 Date: 2008-10-19
Precipitate Volume (nl): 100 Date: 2008-10-19
Reservoir Volume (ul): 50.0 Date: 2008-10-19

Plate: 53775 (S0003849) Cell: XT Date: 2007-10-02 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: A05

Screen Type: Fine Date: 2008-10-19
Setup Date: 2007-10-02 Date: 2010-10-01
Setup Temp: 20 Date: 2008-10-19
Crystal Conditions: S0003849-GNF2-E04 Date: 2008-10-19
Drop Volume (nl): 100.0 Date: 2008-10-19
Precipitate Volume (nl): 100 Date: 2008-10-19
Reservoir Volume (ul): 50.0 Date: 2008-10-19

Plate: 53776 (S0003868) Cell: XT Date: 2007-10-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: A05

Screen Type: Fine Date: 2008-10-19
Setup Date: 2007-10-08 Date: 2010-10-01
Setup Temp: 4 Date: 2008-10-19
Crystal Conditions: S0003868-GNF2-D11 Date: 2008-10-19
Drop Volume (nl): 100.0 Date: 2008-10-19
Precipitate Volume (nl): 100 Date: 2008-10-19
Reservoir Volume (ul): 50.0 Date: 2008-10-19

Plate: 53777 (S0003869) Cell: XT Date: 2007-10-08 Status: Success Previous Location: Plate: 47552 (E-1115) Cell: A05

Screen Type: Fine Date: 2008-10-19
Setup Date: 2007-10-08 Date: 2010-10-01
Setup Temp: 20 Date: 2008-10-19
Crystal Conditions: S0003869-GNF2-D11 Date: 2008-10-19
Drop Volume (nl): 100.0 Date: 2008-10-19
Precipitate Volume (nl): 100 Date: 2008-10-19
Reservoir Volume (ul): 50.0 Date: 2008-10-19

STAGE: Crystal Hit

Plate: 48803 (G0033495) Cell: F01 Date: 2007-06-01 Status: Success Previous Location: Plate: 48803 (G0033495) Cell: XT

Setup Date: 2007-06-01 Date: 2008-10-19
Screen Date: 2007-06-29 Date: 2008-10-19
Screen Conditions: GNF1 F01: 10.0% PEG-6000, 0.1M MES pH 6.0 Date: 2008-10-19
Crystal Classification: CRYSTAL HIT Date: 2008-10-19

Plate: 48804 (G0033357) Cell: B08 Date: 2007-06-01 Status: Success Previous Location: Plate: 48804 (G0033357) Cell: XT

Setup Date: 2007-06-01 Date: 2008-10-19
Screen Date: 2007-06-29 Date: 2008-10-19
Screen Conditions: GNF2 B08: 0.2M MgCl2, 40.0% MPD, 0.1M Imidazole pH 8.0 Date: 2008-10-19
Crystal Classification: CRYSTAL HIT Date: 2008-10-19

Plate: 48804 (G0033357) Cell: C03 Date: 2007-06-01 Status: Success Previous Location: Plate: 48804 (G0033357) Cell: XT

Setup Date: 2007-06-01 Date: 2008-10-19
Screen Date: 2007-06-29 Date: 2008-10-19
Screen Conditions: GNF2 C03: 0.2M MgCl2, 30.0% PEG-400, 0.1M HEPES pH 7.5 Date: 2008-10-19
Crystal Classification: CRYSTAL HIT Date: 2008-10-19

Plate: 48804 (G0033357) Cell: D11 Date: 2007-06-01 Status: Success Previous Location: Plate: 48804 (G0033357) Cell: XT

Setup Date: 2007-06-01 Date: 2008-10-19
Screen Date: 2007-06-29 Date: 2008-10-19
Screen Conditions: GNF2 D11: 12.0% PEG-20000, 0.1M MES pH 6.5 Date: 2008-10-19
Crystal Classification: PROBABLY HARVESTABLE Date: 2008-10-19

Plate: 48804 (G0033357) Cell: E04 Date: 2007-06-01 Status: Success Previous Location: Plate: 48804 (G0033357) Cell: XT

Setup Date: 2007-06-01 Date: 2008-10-19
Screen Date: 2007-06-29 Date: 2008-10-19
Screen Conditions: GNF2 E04: 1.0M LiCl, 10.0% PEG-6000, 0.1M MES pH 6.0 Date: 2008-10-19
Crystal Classification: PROBABLY HARVESTABLE Date: 2008-10-19

Plate: 48806 (G0033259) Cell: B04 Date: 2007-06-01 Status: Success Previous Location: Plate: 48806 (G0033259) Cell: XT

Setup Date: 2007-06-01 Date: 2008-10-19
Screen Date: 2007-06-18 Date: 2008-10-19
Screen Conditions: GNF4 B04: 65.0% MPD, 0.1M Bicine pH 9.0 Date: 2008-10-19
Crystal Classification: CRYSTAL HIT Date: 2008-10-19

Plate: 53774 (S0003843) Cell: D01 Date: 2007-10-02 Status: Success Previous Location: Plate: 53774 (S0003843) Cell: XT

Setup Date: 2007-10-02 Date: 2010-10-31
Screen Date: 2007-10-11 Date: 2010-10-31
Screen Conditions: S0003843-GNF2-E04 D01: 1.00M lithium chloride, 0.1M MES pH 5.93 Date: 2010-10-02
Crystal Classification: CRYSTAL HIT Date: 2008-10-19

Plate: 53776 (S0003868) Cell: A07 Date: 2007-10-08 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: XT

Setup Date: 2007-10-08 Date: 2010-10-31
Screen Date: 2007-10-16 Date: 2010-10-31
Screen Conditions: S0003868-GNF2-D11 A07: 9.300% polyethylene glycol 20000, 0.1M MES pH 6.0 Date: 2010-10-02
Crystal Classification: PROBABLY HARVESTABLE Date: 2008-10-19

Plate: 53776 (S0003868) Cell: A08 Date: 2007-10-08 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: XT

Setup Date: 2007-10-08 Date: 2010-10-31
Screen Date: 2007-10-16 Date: 2010-10-31
Screen Conditions: S0003868-GNF2-D11 A08: 10.500% polyethylene glycol 20000, 0.1M MES pH 6.0 Date: 2010-10-02
Crystal Classification: PROBABLY HARVESTABLE Date: 2008-10-19

Plate: 53776 (S0003868) Cell: B07 Date: 2007-10-08 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: XT

Setup Date: 2007-10-08 Date: 2010-10-31
Screen Date: 2007-10-16 Date: 2010-10-31
Screen Conditions: S0003868-GNF2-D11 B07: 9.300% polyethylene glycol 20000, 0.1M MES pH 6.14 Date: 2010-10-02
Crystal Classification: PROBABLY HARVESTABLE Date: 2008-10-19

Plate: 53776 (S0003868) Cell: B08 Date: 2007-10-08 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: XT

Setup Date: 2007-10-08 Date: 2010-10-31
Screen Date: 2007-10-16 Date: 2010-10-31
Screen Conditions: S0003868-GNF2-D11 B08: 10.500% polyethylene glycol 20000, 0.1M MES pH 6.14 Date: 2010-10-02
Crystal Classification: PROBABLY HARVESTABLE Date: 2008-10-19

Plate: 53776 (S0003868) Cell: B09 Date: 2007-10-08 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: XT

Setup Date: 2007-10-08 Date: 2010-10-31
Screen Date: 2007-10-16 Date: 2010-10-31
Screen Conditions: S0003868-GNF2-D11 B09: 11.600% polyethylene glycol 20000, 0.1M MES pH 6.14 Date: 2010-10-02
Crystal Classification: CRYSTAL HIT Date: 2008-10-19

Plate: 53776 (S0003868) Cell: C09 Date: 2007-10-08 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: XT

Setup Date: 2007-10-08 Date: 2010-10-31
Screen Date: 2007-10-16 Date: 2010-10-31
Screen Conditions: S0003868-GNF2-D11 C09: 11.600% polyethylene glycol 20000, 0.1M MES pH 6.29 Date: 2010-10-02
Crystal Classification: CRYSTAL HIT Date: 2008-10-19

STAGE: SDC Receiving

Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L3 Date: 2007-06-19 Status: Success Previous Location: Plate: 48804 (G0033357) Cell: D11

Shipment ID: 1282 Date: 2007-06-21

Cryo Conditions: 10% Ethylene-Glycol Date: 2007-06-21
Crystal Comments: rod Date: 2007-06-21
Ship Date: 2007-06-19 Date: 2007-06-21
Crystal Id: 55061 Date: 2007-06-21
Pin Position: 7222 Date: 2007-06-21
Ship Position: SSRL 00198 Date: 2007-06-21
Mount Date: 2007-06-19 Date: 2007-06-21

Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L4 Date: 2007-06-19 Status: Success Previous Location: Plate: 48804 (G0033357) Cell: D11

Shipment ID: 1282 Date: 2007-06-21

Cryo Conditions: 10% Ethylene-Glycol Date: 2007-06-21
Crystal Comments: rod Date: 2007-06-21
Ship Date: 2007-06-19 Date: 2007-06-21
Crystal Id: 55062 Date: 2007-06-21
Pin Position: 3252 Date: 2007-06-21
Ship Position: SSRL 00198 Date: 2007-06-21
Mount Date: 2007-06-19 Date: 2007-06-21

Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L5 Date: 2007-06-19 Status: Success Previous Location: Plate: 48804 (G0033357) Cell: D11

Shipment ID: 1282 Date: 2007-06-21

Cryo Conditions: 10% Ethylene-Glycol Date: 2007-06-21
Crystal Comments: rod Date: 2007-06-21
Ship Date: 2007-06-19 Date: 2007-06-21
Crystal Id: 55063 Date: 2007-06-21
Pin Position: 6060 Date: 2007-06-21
Ship Position: SSRL 00198 Date: 2007-06-21
Mount Date: 2007-06-19 Date: 2007-06-21

Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L6 Date: 2007-06-19 Status: Success Previous Location: Plate: 48804 (G0033357) Cell: E04

Shipment ID: 1282 Date: 2007-06-21

Cryo Conditions: 20% Ethylene-Glycol Date: 2007-06-21
Crystal Comments: rod Date: 2007-06-21
Ship Date: 2007-06-19 Date: 2007-06-21
Crystal Id: 55064 Date: 2007-06-21
Pin Position: 7558 Date: 2007-06-21
Ship Position: SSRL 00198 Date: 2007-06-21
Stop Flag: 0 Date: 2008-04-10
SDC Stage: 2900 Date: 2008-04-10
Mount Date: 2007-06-19 Date: 2007-06-21

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: G6 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: A07

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 10% Glycerol Date: 2007-11-01
Crystal Comments: small cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66947 Date: 2007-11-01
Pin Position: 1636 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: G7 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: A07

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 10% Glycerol Date: 2007-11-01
Crystal Comments: small cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66948 Date: 2007-11-01
Pin Position: 1630 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: G8 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: A07

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 10% Glycerol Date: 2007-11-01
Crystal Comments: small cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66949 Date: 2007-11-01
Pin Position: 1600 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H1 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: A08

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% Ethylene-Glycol Date: 2007-11-01
Crystal Comments: small cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66950 Date: 2007-11-01
Pin Position: 1532 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H2 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: A08

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% Ethylene-Glycol Date: 2007-11-01
Crystal Comments: small cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66951 Date: 2007-11-01
Pin Position: 1620 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H3 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: A08

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% Ethylene-Glycol Date: 2007-11-01
Crystal Comments: small cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66952 Date: 2007-11-01
Pin Position: 1608 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H4 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: A08

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% Ethylene-Glycol Date: 2007-11-01
Crystal Comments: small cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66953 Date: 2007-11-01
Pin Position: 1834 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H5 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: B07

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% Glycerol Date: 2007-11-01
Crystal Comments: cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66954 Date: 2007-11-01
Pin Position: 195 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H6 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: B07

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% Glycerol Date: 2007-11-01
Crystal Comments: cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66955 Date: 2007-11-01
Pin Position: 1245 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H7 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: B07

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% Glycerol Date: 2007-11-01
Crystal Comments: cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66956 Date: 2007-11-01
Pin Position: 925 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H8 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: B07

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% Glycerol Date: 2007-11-01
Crystal Comments: cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66957 Date: 2007-11-01
Pin Position: 1495 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: I1 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: B08

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% PEG-600 Date: 2007-11-01
Crystal Comments: cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66958 Date: 2007-11-01
Pin Position: 9512 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: I2 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: B08

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% PEG-600 Date: 2007-11-01
Crystal Comments: cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66959 Date: 2007-11-01
Pin Position: 884 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: I3 Date: 2007-10-30 Status: Success Previous Location: Plate: 53776 (S0003868) Cell: B08

Shipment ID: 1425 Date: 2007-11-01

Cryo Conditions: 20% PEG-600 Date: 2007-11-01
Crystal Comments: cube Date: 2007-11-01
Ship Date: 2007-10-30 Date: 2007-11-01
Crystal Id: 66960 Date: 2007-11-01
Pin Position: 7773 Date: 2007-11-01
Ship Position: SSRL 00269 Date: 2007-11-01
Mount Date: 2007-10-30 Date: 2007-11-01

STAGE: Screening

Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L3 Date: 2007-06-22 Status: Success

Image Directory: /data/jcsg/ssrl3/1_5/20070620/screening/cassette198/HP10638A Date: 2007-06-30
Xtal Quality: 1 Date: 2007-06-30
Ice Rings: 8 Date: 2007-06-30
Diffraction Quality: 1 Date: 2007-06-30
Single Xtal: 1 Date: 2007-06-30
Spot Shape: 7 Date: 2007-06-30
Maximum Resolution: 10 Date: 2007-06-30
Beamline: SSRL BL1-5 Date: 2007-06-30
Robot Y or N: 1 Date: 2007-06-30
Screen Date: 2007-06-22 Date: 2007-06-30
Freezing: FROST Date: 2007-06-30
Loop: OK Date: 2007-06-30
Crystal Condition: CND Date: 2007-06-30

Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L4 Date: 2007-06-22 Status: No Diffraction

Image Directory: /data/jcsg/ssrl3/1_5/20070620/screening/cassette198/HP10638A Date: 2007-06-30
Xtal Quality: 0 Date: 2007-06-30
Ice Rings: 8 Date: 2007-06-30
Diffraction Quality: 0 Date: 2007-06-30
Beamline: SSRL BL1-5 Date: 2007-06-30
Robot Y or N: 1 Date: 2007-06-30
Screen Date: 2007-06-22 Date: 2007-06-30
Freezing: FROST Date: 2007-06-30
Loop: OK Date: 2007-06-30
Crystal Condition: CND Date: 2007-06-30

Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L5 Date: 2007-06-22 Status: Success

Image Directory: /data/jcsg/ssrl3/1_5/20070620/screening/cassette198/HP10638A Date: 2007-06-30
Xtal Quality: 1 Date: 2007-06-30
Ice Rings: 8 Date: 2007-06-30
Diffraction Quality: 1 Date: 2007-06-30
Single Xtal: ? Date: 2007-06-30
Spot Shape: 6 Date: 2007-06-30
Maximum Resolution: 31 Date: 2007-06-30
Beamline: SSRL BL1-5 Date: 2007-06-30
Robot Y or N: 1 Date: 2007-06-30
Screen Date: 2007-06-22 Date: 2007-06-30
Freezing: FROST Date: 2007-06-30
Loop: OK Date: 2007-06-30
Crystal Condition: SINGLE Date: 2007-06-30

Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L6 Date: 2007-06-22 Status: Success

Image Directory: /data/jcsg/ssrl3/1_5/20070620/screening/cassette198/HP10638A Date: 2010-01-28
Xtal Quality: 6 Date: 2010-01-28
Ice Rings: 0 Date: 2010-01-28
Diffraction Quality: 10 Date: 2010-01-28
Single Xtal: 1 Date: 2010-01-28
Spot Shape: 6 Date: 2010-01-28
Maximum Resolution: 2 Date: 2010-01-28
Beamline: SSRL BL1-5 Date: 2010-01-28
Robot Y or N: 1 Date: 2010-01-28
Screen Date: 2007-06-22 Date: 2010-01-28
Freezing: CLEAR Date: 2010-01-28
Loop: OK Date: 2010-01-28
Crystal Condition: CND Date: 2010-01-28
Comments: crystal lost on 2009/07/20 Date: 2010-01-28

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: G6 Date: 2007-11-04 Status: No Diffraction

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 0 Date: 2007-11-09
Ice Rings: 8 Date: 2007-11-09
Diffraction Quality: 0 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: G7 Date: 2007-11-04 Status: No Diffraction

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 0 Date: 2007-11-09
Ice Rings: 5 Date: 2007-11-09
Diffraction Quality: 0 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: G8 Date: 2007-11-04 Status: Success

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 1 Date: 2007-11-09
Ice Rings: 1 Date: 2007-11-09
Diffraction Quality: 3 Date: 2007-11-09
Single Xtal: ? Date: 2007-11-09
Spot Shape: 7 Date: 2007-11-09
Maximum Resolution: 10 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H1 Date: 2007-11-04 Status: Success

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 1 Date: 2007-11-09
Ice Rings: 0 Date: 2007-11-09
Diffraction Quality: 3 Date: 2007-11-09
Single Xtal: ? Date: 2007-11-09
Spot Shape: 7 Date: 2007-11-09
Maximum Resolution: 11 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H2 Date: 2007-11-04 Status: Success

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 1 Date: 2007-11-09
Ice Rings: 5 Date: 2007-11-09
Diffraction Quality: 3 Date: 2007-11-09
Single Xtal: ? Date: 2007-11-09
Spot Shape: 7 Date: 2007-11-09
Maximum Resolution: 18 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: SINGLE Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H3 Date: 2007-11-04 Status: Success

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 1 Date: 2007-11-09
Ice Rings: 0 Date: 2007-11-09
Diffraction Quality: 6 Date: 2007-11-09
Single Xtal: ? Date: 2007-11-09
Spot Shape: 8 Date: 2007-11-09
Maximum Resolution: 11 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H4 Date: 2007-11-04 Status: Success

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 1 Date: 2007-11-09
Ice Rings: 0 Date: 2007-11-09
Diffraction Quality: 4 Date: 2007-11-09
Single Xtal: ? Date: 2007-11-09
Spot Shape: 8 Date: 2007-11-09
Maximum Resolution: 10 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H5 Date: 2007-11-04 Status: Success

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 2 Date: 2007-11-09
Ice Rings: 8 Date: 2007-11-09
Diffraction Quality: 4 Date: 2007-11-09
Single Xtal: ? Date: 2007-11-09
Spot Shape: 7 Date: 2007-11-09
Maximum Resolution: 8 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: FROST Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H6 Date: 2007-11-04 Status: No Diffraction

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 0 Date: 2007-11-09
Ice Rings: 8 Date: 2007-11-09
Diffraction Quality: 0 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H7 Date: 2007-11-04 Status: No Diffraction

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 0 Date: 2007-11-09
Ice Rings: 8 Date: 2007-11-09
Diffraction Quality: 0 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: FROST Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: H8 Date: 2007-11-04 Status: Success

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 1 Date: 2007-11-09
Ice Rings: 8 Date: 2007-11-09
Diffraction Quality: 4 Date: 2007-11-09
Single Xtal: ? Date: 2007-11-09
Spot Shape: 8 Date: 2007-11-09
Maximum Resolution: 30 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: I1 Date: 2007-11-04 Status: No Diffraction

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 0 Date: 2007-11-09
Ice Rings: 8 Date: 2007-11-09
Diffraction Quality: 0 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: FROST Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: I2 Date: 2007-11-04 Status: Success

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 4 Date: 2007-11-09
Ice Rings: 0 Date: 2007-11-09
Diffraction Quality: 9 Date: 2007-11-09
Single Xtal: ? Date: 2007-11-09
Spot Shape: 7 Date: 2007-11-09
Maximum Resolution: 4.8 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: CLEAR Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

Dewar: 54569 (Storm-10/30/2007) Cassette: SSRL 00269 (SSRL 00269Storm-10/30/2007) Cell: I3 Date: 2007-11-04 Status: No Diffraction

Image Directory: /data/jcsg/ssrl3/micro2/20071031/screening/cassette269/HP10638A Date: 2007-11-09
Xtal Quality: 0 Date: 2007-11-09
Ice Rings: 8 Date: 2007-11-09
Diffraction Quality: 0 Date: 2007-11-09
Beamline: SSRL MICR2 Date: 2007-11-09
Robot Y or N: 1 Date: 2007-11-09
Screen Date: 2007-11-04 Date: 2007-11-09
Freezing: FROST Date: 2007-11-09
Loop: OK Date: 2007-11-09
Crystal Condition: CND Date: 2007-11-09

STAGE: Data Collection

Collection: 58603 Cell: 1 Date: 2008-02-01 Status: Success Previous Location: Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L6

CollectData.xml: CollectData_2.xml Date: 2008-02-12
Beamline.xml: beamline_2.xml Date: 2008-02-12
Notes file: notes_2.txt Date: 2008-02-12
Beamline: BL11-1 Date: 2008-02-12
ExperimentType: X-Ray Date: 2008-02-12
Program name: Blu-Ice Date: 2008-02-12
Program version: 4.0 Date: 2008-02-12
Synchrotron: SSRL Date: 2008-02-12
Detector name: MAR325 Date: 2008-02-12
Detector type: CCD Date: 2008-02-12
Detector serial: 20 Date: 2008-02-12
Detector manufacturer: MAR Date: 2008-02-12
BeamlineInfoURL: http://biosync.sdsc.edu/ssrl/BL11-1.html Date: 2008-02-12
MOSFLM_DETECTOR_PARAMETERS: !commented out due to use of new mosflm !DETECTOR ROTATION HORIZ ANTI FAST HORIZONTAL ORIGIN UR rectangular TYPE MARCCD !SIZE 4096 4096 HEADER 1 byte 4096 !LIMITS XMIN 0 XMAX 162.5 YMIN 0 YMAX 162.5 xscan 2048 yscan 2048 GAIN 0.31 Date: 2008-02-12
MOSFLM_BEAMLINE_PARAMETERS: SYNCHROTRON POLARIZATION 0.92 DIVERGENCE 0.250 0.030 DISPERSION 0.00020 Date: 2008-02-12
DC Date: 2008-02-01 Date: 2008-02-12
Performed By: Hsiu-Ju (Jessica) Chiu Date: 2008-02-12
Temperature (K): 100 Date: 2008-02-12
Beamline: BL11-1 Date: 2008-02-12
ImageDirectory: /data/jcsg/ssrl3/11_1/20080131/collection/HP10638A_2/55064/ Date: 2008-02-12
Description: d2l1s1 Date: 2008-02-12
Wavelength: 0.97859 Date: 2008-02-12
Anomalous fprimv: -7.12 Date: 2008-02-12
Anomalous fprprv: 5.2 Date: 2008-02-12
DetectorMode: undefined Date: 2008-02-12
Distance: 750.00 Date: 2008-02-12
BeamCenter X: 312.5 Date: 2008-02-12
BeamCenter Y: 162.5 Date: 2008-02-12
ImagePrefix: 55064_1 Date: 2008-02-12
ImageSuffix: mccd Date: 2008-02-12
PhiDelta: 0.10 Date: 2008-02-12
PhiStart: 132.00 Date: 2008-02-12
FrameNumber start: 1 Date: 2008-02-12
FrameNumber end: 914 Date: 2008-02-12

Collection: 58603 Cell: 2 Date: 2008-02-01 Status: Success Previous Location: Dewar: 49480 (Lynx-06/19/2007) Cassette: SSRL 00198 (SSRL 00198Lynx-06/19/2007) Cell: L6

CollectData.xml: CollectData_2.xml Date: 2008-02-12
Beamline.xml: beamline_2.xml Date: 2008-02-12
Notes file: notes_2.txt Date: 2008-02-12
Beamline: BL11-1 Date: 2008-02-12
ExperimentType: X-Ray Date: 2008-02-12
Program name: Blu-Ice Date: 2008-02-12
Program version: 4.0 Date: 2008-02-12
Synchrotron: SSRL Date: 2008-02-12
Detector name: MAR325 Date: 2008-02-12
Detector type: CCD Date: 2008-02-12
Detector serial: 20 Date: 2008-02-12
Detector manufacturer: MAR Date: 2008-02-12
BeamlineInfoURL: http://biosync.sdsc.edu/ssrl/BL11-1.html Date: 2008-02-12
MOSFLM_DETECTOR_PARAMETERS: !commented out due to use of new mosflm !DETECTOR ROTATION HORIZ ANTI FAST HORIZONTAL ORIGIN UR rectangular TYPE MARCCD !SIZE 4096 4096 HEADER 1 byte 4096 !LIMITS XMIN 0 XMAX 162.5 YMIN 0 YMAX 162.5 xscan 2048 yscan 2048 GAIN 0.31 Date: 2008-02-12
MOSFLM_BEAMLINE_PARAMETERS: SYNCHROTRON POLARIZATION 0.92 DIVERGENCE 0.250 0.030 DISPERSION 0.00020 Date: 2008-02-12
DC Date: 2008-02-01 Date: 2008-02-12
Performed By: Hsiu-Ju (Jessica) Chiu Date: 2008-02-12
Temperature (K): 100 Date: 2008-02-12
Beamline: BL11-1 Date: 2008-02-12
ImageDirectory: /data/jcsg/ssrl3/11_1/20080131/collection/HP10638A_2/55064/ Date: 2008-02-12
Description: d2l1s2 Date: 2008-02-12
Wavelength: 0.97859 Date: 2008-02-12
Anomalous fprimv: -7.12 Date: 2008-02-12
Anomalous fprprv: 5.2 Date: 2008-02-12
DetectorMode: undefined Date: 2008-02-12
Distance: 750.00 Date: 2008-02-12
BeamCenter X: 312.5 Date: 2008-02-12
BeamCenter Y: 162.5 Date: 2008-02-12
ImagePrefix: 55064_2 Date: 2008-02-12
ImageSuffix: mccd Date: 2008-02-12
PhiDelta: 0.10 Date: 2008-02-12
PhiStart: 222.00 Date: 2008-02-12
FrameNumber start: 1 Date: 2008-02-12
FrameNumber end: 900 Date: 2008-02-12

STAGE: Integration

Collection: 58604 Cell: 1 Date: 2008-02-12 Status: Success Previous Location: Collection: 58603 Cell: 1

Integration Log File: INTEGRATE.LP Date: 2008-02-12
Integration Reflection File: INTEGRATE.HKL Date: 2008-02-12
Integration Name: l1s1i Date: 2008-02-12
Integration Comments: Very long axis: 83.8400 83.8400 682.3100 90.0000 90.0000 120.0000 SG: P6122, 3 mols/asu. 180 degrees of data collected in two sets with 0.1 degree oscillations by moving detector and keeping distance at 750 mm. Date: 2008-02-12
Performed By: 2912 Date: 2008-02-12
Integration Date: 2008-02-12 Date: 2008-02-12

Collection: 58604 Cell: 2 Date: 2008-02-12 Status: Success Previous Location: Collection: 58603 Cell: 2

Integration Log File: INTEGRATE.LP Date: 2008-02-12
Integration Reflection File: INTEGRATE.HKL Date: 2008-02-12
Integration Name: l1s2i Date: 2008-02-12
Performed By: 2912 Date: 2008-02-12
Integration Date: 2008-02-12 Date: 2008-02-12

STAGE: Scaling

Collection: 58605 Cell: 1 Date: 2008-02-12 Status: Success Previous Location: Collection: 58604 Cell: 1 Collection: 58604 Cell: 2

Scale Log File: XSCALE.LP Date: 2008-04-10
Scale Reflection File: scala.mtz Date: 2008-04-10
Scale Name: l1s Date: 2008-04-10
Scale Comments: Integration, Scaling, Truncate done by xsolve. XDS/XSCALE used. Files uploaded from /dataj4/jcsg/xtal_process5/HP10638A/55064_2/try1/scale_xds/p622/lambda1/manual to make the cell dimensions+resolution limit match the output in truncate tab. Date: 2008-04-10
Performed By: 2912 Date: 2008-04-10
Scaling program: XSCALE Date: 2008-04-10
Program Version: December 6 Date: 2008-04-10
Space group: P622 Date: 2008-02-12
Cell A: 83.84 Date: 2008-04-10
Cell B: 83.84 Date: 2008-04-10
Cell C: 682.31 Date: 2008-04-10
Angle Alpha: 90.000 Date: 2008-04-10
Angle Beta: 90.000 Date: 2008-04-10
Angle Gamma: 120.000 Date: 2008-04-10
High resolution overall: 2.39 Date: 2008-04-10
Low resolution overall: 10.00 Date: 2008-04-10
Rejection criteria sigma(I) overall: -3.0 Date: 2008-04-10
Completeness overall: 88.6% Date: 2008-04-10
Redundancy overall: 4.4441852751415 Date: 2008-04-10
Rsym(I) overall: 5.5% Date: 2008-04-10
I/sigma(I) overall: 16.87 Date: 2008-04-10
High resolution limit for highest resolution shell: 2.39 Date: 2008-04-10
Low resolution limit for highest resolution shell: 2.60 Date: 2008-04-10
Completeness shell: 73.5% Date: 2008-04-10
Redundancy shell: 3.11944362237348 Date: 2008-04-10
Rsym(I) shell: 61.6% Date: 2008-04-10
I/sigma(I) shell: 2.27 Date: 2008-04-10
Observed reflections overall: 415358 Date: 2008-04-10
Unmerged HKL File: SCALEPACK Date: 2008-04-10
Scaling Date: 2008-02-12 Date: 2008-04-10
Number of reflections: 415358 Date: 2008-04-10

STAGE: Truncating

Collection: 58606 Cell: 1 Date: 2008-02-12 Status: Success Previous Location: Collection: 58605 Cell: 1

Truncate Name: l1t Date: 2008-02-12
Performed By: 2912 Date: 2008-02-12
Truncate Log File: truncate.log Date: 2008-02-12
Truncate Reflection File: truncate.mtz Date: 2008-02-12
truncate program version: 5.0 Date: 2008-02-12
Unique reflections overall: 1248528 Date: 2008-02-12
Wilson_Bfact: 58.480 Date: 2008-02-12
Truncate Date: 2008-02-12 Date: 2008-02-12
Cell A: 83.8400 Date: 2008-02-12
Cell B: 83.8400 Date: 2008-02-12
Cell C: 682.3100 Date: 2008-02-12
Angle Alpha: 90.0000 Date: 2008-02-12
Angle Beta: 90.0000 Date: 2008-02-12
Angle Gamma: 120.0000 Date: 2008-02-12
Unique Reflections: 52024 Date: 2008-02-12
Resolution Range Low: 29.656 Date: 2008-02-12
Resolution Range High: 2.395 Date: 2008-02-12

STAGE: Phasing

Collection: 58607 Cell: 1 Date: 2008-02-12 Status: Success Previous Location: Collection: 58606 Cell: 1

Name of phasing run: l1p Date: 2008-02-12
Performed By: 2912 Date: 2008-02-12
Phasing Comments: Sites were found using HySS and SHELXD (from 15-3A) from data originally processed to 2.7A using HKL2000. Could not find reliable sites with the XDS processed data to 2.4A in the 15-3A range. Could be due to some data reduction differences. Finally, just used the first set of sites found with the HKL2000 processed data and obtained phases to 2.4A using the XDS processed data and SOLVE. Phasing information uploaded from /home/debanu/dd_jcsg/HP10638A_55064_2_solve Date: 2008-02-12
Phasing program: SOLVE Date: 2008-02-12
Phasing reflection file: solve.mtz Date: 2008-02-12
Phasing PRT log: ha.pdb Date: 2008-02-12
Phasing log file: solve.prt Date: 2008-02-12
Solution Type: SAD Date: 2008-02-12
Phasing Date: 2008-02-12 Date: 2008-02-12

STAGE: Density Modification

Collection: 58610 Cell: 1 Date: 2008-02-12 Status: Success Previous Location: Collection: 58606 Cell: 1 Collection: 58607 Cell: 1

Name of DM run: l1dm Date: 2008-02-12
Performed By: 2912 Date: 2008-02-12
DM Comments: Density modification with RESOLVE. Files uploaded from /home/debanu/dd_jcsg/HP10638A_55064_2_solve Date: 2008-02-12
DM reflection file: resolve.mtz Date: 2008-02-12
DM log file: resolve.log Date: 2008-02-12
DM Date: 2008-02-12 Date: 2008-02-12

STAGE: Tracing

Collection: 58611 Cell: 1 Date: 2008-02-12 Status: Solved Structure Previous Location: Collection: 58606 Cell: 1 Collection: 58610 Cell: 1 Collection: 58607 Cell: 1

Name of tracing run: l1trace Date: 2008-02-12
Performed By: 2912 Date: 2008-02-12
Tracing Comments: Traced using ARP/WARP with density modified experimental phase file resolve.mtz. ~84% main chain/~75% side chain traced. Chains have to be reorganized during refinement. Files uploaded from /home/debanu/dd_jcsg/HP10638A_55064_2_solve Date: 2008-02-12
Tracing program: CCP4 - Arp Warp Date: 2008-02-12
Tracing log file: 4_arp_warp.log Date: 2008-02-12
Tracing reflection file: 4_warpNtrace.mtz Date: 2008-02-12
Tracing PDB: 4_warpNtrace.pdb Date: 2008-02-12
Tracing Program Version: 1.3.9 Date: 2008-02-12
Tracing Date: 2008-02-12 Date: 2008-02-12

STAGE: Refining

Collection: 59465 Cell: 1 Date: 2008-03-03 Status: Success Previous Location: Collection: 58611 Cell: 1 Collection: 58606 Cell: 1 Collection: 58610 Cell: 1 Collection: 58607 Cell: 1

Name of refinement run: Ref1 Date: 2008-03-03
Performed By: 2912 Date: 2008-03-03
Refinement Comments: See attached notes. Date: 2008-03-03
REFINEMENT TARGET :: MAXIMUM LIKELIHOOD WITH PHASES Date: 2008-03-03
RESOLUTION RANGE HIGH:: 2.39 Date: 2008-03-03
DATA CUTOFF (SIGMA(F)) :: NONE Date: 2008-03-03
COMPLETENESS (WORKING+TEST) (%) :: 89.28 Date: 2008-03-03
NUMBER OF REFLECTIONS :: 49260 Date: 2008-03-03
CROSS-VALIDATION METHOD :: THROUGHOUT Date: 2008-03-03
FREE R VALUE TEST SET SELECTION :: RANDOM Date: 2008-03-03
R VALUE (WORKING SET) :: 0.19519 Date: 2008-03-03
FREE R VALUE :: 0.22330 Date: 2008-03-03
FREE R VALUE TEST SET SIZE (%) :: 5.1 Date: 2008-03-03
FREE R VALUE TEST SET COUNT :: 2650 Date: 2008-03-03
TOTAL NUMBER OF BINS USED :: 20 Date: 2008-03-03
BIN RESOLUTION RANGE HIGH (A) :: 2.395 Date: 2008-03-03
BIN RESOLUTION RANGE LOW (A) :: 2.457 Date: 2008-03-03
REFLECTIONS IN BIN (WORKING SET) :: 2839 Date: 2008-03-03
BIN R VALUE (WORKING SET) :: 0.387 Date: 2008-03-03
BIN FREE R VALUE :: 0.430 Date: 2008-03-03
BIN FREE R VALUE TEST SET COUNT :: 153 Date: 2008-03-03
MEAN B VALUE (OVERALL, A**2) :: 35.780 Date: 2008-03-03
OVERALL ANISOTROPIC B VALUE B11 (A**2) :: 3.42 Date: 2008-03-03
OVERALL ANISOTROPIC B VALUE B22 (A**2) :: 3.42 Date: 2008-03-03
OVERALL ANISOTROPIC B VALUE B33 (A**2) :: -5.13 Date: 2008-03-03
OVERALL ANISOTROPIC B VALUE B12 (A**2) :: 1.71 Date: 2008-03-03
OVERALL ANISOTROPIC B VALUE B13 (A**2) :: 0.00 Date: 2008-03-03
OVERALL ANISOTROPIC B VALUE B23 (A**2) :: 0.00 Date: 2008-03-03
SPACE GROUP: P6122 Date: 2008-03-03
MAIN-CHAIN ANGLE RMS (A**2) :: 1.373 Date: 2008-03-03
BOND LENGTHS RMS DEVIATIONS (A) :: 0.008 Date: 2008-03-03
Refinement Log: 75_refmac5.log Date: 2008-03-03
Reflection File: 55064_refmac20.mtz Date: 2008-03-03
PDB file: 55064_refmac20.pdb Date: 2008-03-03
Program: REFMAC Date: 2008-03-03
Version: 5.4.0067 Date: 2008-03-03
Cell A: 83.840 Date: 2008-03-03
Cell B: 83.840 Date: 2008-03-03
Cell C: 682.310 Date: 2008-03-03
Angle Alpha: 90.00 Date: 2008-03-03
Angle Beta: 90.00 Date: 2008-03-03
Angle Gamma: 120.00 Date: 2008-03-03
ALL ATOMS: 7586 Date: 2008-03-03
ESU BASED ON R VALUE: 0.313 Date: 2008-03-03
ESU BASED ON FREE R VALUE: 0.222 Date: 2008-03-03
ESU BASED ON MAXIMUM LIKELIHOOD: 0.151 Date: 2008-03-03
ESU FOR B VALUE BASED ON MAXIMUM LIKELIHOOD: 12.821 Date: 2008-03-03
CORRELATION COEFFICIENT FO-FC: 0.953 Date: 2008-03-03
CORRELATION COEFFICIENT FO-FC FREE: 0.940 Date: 2008-03-03
BOND LENGTHS REFINED ATOMS - LENGTH COUNT: 7553 Date: 2008-03-03
BOND LENGTHS REFINED ATOMS - LENGTH WEIGHT: 0.022 Date: 2008-03-03
BOND ANGLES REFINED ATOMS - ANGLE WEIGHT: 1.949 Date: 2008-03-03
CHIRAL-CENTER RESTRAINTS RMS: 0.063 Date: 2008-03-03
TORSION ANGLES, PERIOD COUNT: 928::360::1200::42 Date: 2008-03-03
TORSION ANGLES, PERIOD RMS: 6.668::33.450::16.926::15.201 Date: 2008-03-03
TORSION ANGLES, PERIOD WEIGHT: 5.000::24.139::15.000::15.000 Date: 2008-03-03
CHIRAL-CENTER RESTRAINTS COUNT: 1102 Date: 2008-03-03
CHIRAL-CENTER RESTRAINTS WEIGHT: 0.200 Date: 2008-03-03
GENERAL PLANES REFINED ATOMS COUNT: 8482 Date: 2008-03-03
GENERAL PLANES REFINED ATOMS RMS: 0.005 Date: 2008-03-03
GENERAL PLANES REFINED ATOMS WEIGHT: 0.021 Date: 2008-03-03
MAIN-CHAIN BOND REFINED ATOMS COUNT: 4608 Date: 2008-03-03
MAIN-CHAIN BOND REFINED ATOMS RMS: 0.504 Date: 2008-03-03
MAIN-CHAIN BOND REFINED ATOMS WEIGHT: 3.000 Date: 2008-03-03
MAIN-CHAIN ANGLE REFINED ATOMS COUNT: 7421 Date: 2008-03-03
MAIN-CHAIN ANGLE REFINED ATOMS RMS: 0.935 Date: 2008-03-03
MAIN-CHAIN ANGLE REFINED ATOMS WEIGHT: 4.000 Date: 2008-03-03
SIDE-CHAIN BOND REFINED ATOMS COUNT: 2945 Date: 2008-03-03
SIDE-CHAIN BOND REFINED ATOMS RMS: 1.829 Date: 2008-03-03
SIDE-CHAIN BOND REFINED ATOMS WEIGHT: 6.000 Date: 2008-03-03
SIDE-CHAIN ANGLE REFINED ATOMS COUNT: 2830 Date: 2008-03-03
SIDE-CHAIN ANGLE REFINED ATOMS RMS: 2.656 Date: 2008-03-03
SIDE-CHAIN ANGLE REFINED ATOMS WEIGHT: 9.000 Date: 2008-03-03
METHOD USED: MASK Date: 2008-03-03
VDW PROBE RADIUS: 1.20 Date: 2008-03-03
ION PROBE RADIUS: 0.80 Date: 2008-03-03
SHRINKAGE RADIUS: 0.80 Date: 2008-03-03
Refinement Date : 2008-03-03 Date: 2008-03-03
Refinement Technical Note: qc_check_HP10638A_030308.html Date: 2008-03-03
Structure Refinement Summary: HP10638A_Xtal55064_030308.doc Date: 2008-03-03
Matthews Coef: 2.02 Date: 2009-07-12
Solvent Percentage: 39.03 Date: 2009-07-12

Collection: 59465 Cell: 2 Date: 2008-03-25 Status: Success Previous Location: Collection: 58611 Cell: 1 Collection: 58606 Cell: 1 Collection: 58610 Cell: 1 Collection: 58607 Cell: 1 Collection: 59465 Cell: 1

Name of refinement run: Ref2 Date: 2008-04-01
Performed By: 2912 Date: 2008-04-01
Refinement Comments: Addressed QC1 comments and suggestions. Date: 2008-04-01
REFINEMENT TARGET :: MAXIMUM LIKELIHOOD WITH PHASES Date: 2008-04-01
RESOLUTION RANGE HIGH:: 2.39 Date: 2008-04-01
DATA CUTOFF (SIGMA(F)) :: NONE Date: 2008-04-01
COMPLETENESS (WORKING+TEST) (%) :: 89.28 Date: 2008-04-01
NUMBER OF REFLECTIONS :: 49260 Date: 2008-04-01
CROSS-VALIDATION METHOD :: THROUGHOUT Date: 2008-04-01
FREE R VALUE TEST SET SELECTION :: RANDOM Date: 2008-04-01
R VALUE (WORKING SET) :: 0.18738 Date: 2008-04-01
FREE R VALUE :: 0.22326 Date: 2008-04-01
FREE R VALUE TEST SET SIZE (%) :: 5.1 Date: 2008-04-01
FREE R VALUE TEST SET COUNT :: 2650 Date: 2008-04-01
TOTAL NUMBER OF BINS USED :: 20 Date: 2008-04-01
BIN RESOLUTION RANGE HIGH (A) :: 2.395 Date: 2008-04-01
BIN RESOLUTION RANGE LOW (A) :: 2.457 Date: 2008-04-01
REFLECTIONS IN BIN (WORKING SET) :: 2839 Date: 2008-04-01
BIN R VALUE (WORKING SET) :: 0.385 Date: 2008-04-01
BIN FREE R VALUE :: 0.408 Date: 2008-04-01
BIN FREE R VALUE TEST SET COUNT :: 153 Date: 2008-04-01
MEAN B VALUE (OVERALL, A**2) :: 35.947 Date: 2008-04-01
OVERALL ANISOTROPIC B VALUE B11 (A**2) :: 3.40 Date: 2008-04-01
OVERALL ANISOTROPIC B VALUE B22 (A**2) :: 3.40 Date: 2008-04-01
OVERALL ANISOTROPIC B VALUE B33 (A**2) :: -5.10 Date: 2008-04-01
OVERALL ANISOTROPIC B VALUE B12 (A**2) :: 1.70 Date: 2008-04-01
OVERALL ANISOTROPIC B VALUE B13 (A**2) :: 0.00 Date: 2008-04-01
OVERALL ANISOTROPIC B VALUE B23 (A**2) :: 0.00 Date: 2008-04-01
SPACE GROUP: P6122 Date: 2008-04-01
MAIN-CHAIN ANGLE RMS (A**2) :: 1.818 Date: 2008-04-01
BOND LENGTHS RMS DEVIATIONS (A) :: 0.016 Date: 2008-04-01
Refinement Log: 90_refmac5.log Date: 2008-03-25
Reflection File: 55064_refmac24.mtz Date: 2008-03-25
PDB file: 55064_refmac24-modified040108.pdb Date: 2008-04-01
Program: REFMAC Date: 2008-04-01
Version: 5.4.0067 Date: 2008-04-01
Cell A: 83.840 Date: 2008-04-01
Cell B: 83.840 Date: 2008-04-01
Cell C: 682.310 Date: 2008-04-01
Angle Alpha: 90.00 Date: 2008-04-01
Angle Beta: 90.00 Date: 2008-04-01
Angle Gamma: 120.00 Date: 2008-04-01
ALL ATOMS: 7728 Date: 2008-04-01
ESU BASED ON R VALUE: 0.308 Date: 2008-04-01
ESU BASED ON FREE R VALUE: 0.224 Date: 2008-04-01
ESU BASED ON MAXIMUM LIKELIHOOD: 0.150 Date: 2008-04-01
ESU FOR B VALUE BASED ON MAXIMUM LIKELIHOOD: 12.494 Date: 2008-04-01
CORRELATION COEFFICIENT FO-FC: 0.957 Date: 2008-04-01
CORRELATION COEFFICIENT FO-FC FREE: 0.939 Date: 2008-04-01
BOND LENGTHS REFINED ATOMS - LENGTH COUNT: 7581 Date: 2008-04-01
BOND LENGTHS REFINED ATOMS - LENGTH WEIGHT: 0.022 Date: 2008-04-01
BOND ANGLES REFINED ATOMS - ANGLE WEIGHT: 1.948 Date: 2008-04-01
CHIRAL-CENTER RESTRAINTS RMS: 0.099 Date: 2008-04-01
TORSION ANGLES, PERIOD COUNT: 932::363::1203::42 Date: 2008-04-01
TORSION ANGLES, PERIOD RMS: 4.216::30.862::14.260::12.584 Date: 2008-04-01
TORSION ANGLES, PERIOD WEIGHT: 5.000::24.132::15.000::15.000 Date: 2008-04-01
CHIRAL-CENTER RESTRAINTS COUNT: 1105 Date: 2008-04-01
CHIRAL-CENTER RESTRAINTS WEIGHT: 0.200 Date: 2008-04-01
GENERAL PLANES REFINED ATOMS COUNT: 8519 Date: 2008-04-01
GENERAL PLANES REFINED ATOMS RMS: 0.008 Date: 2008-04-01
GENERAL PLANES REFINED ATOMS WEIGHT: 0.021 Date: 2008-04-01
MAIN-CHAIN BOND REFINED ATOMS COUNT: 4621 Date: 2008-04-01
MAIN-CHAIN BOND REFINED ATOMS RMS: 0.776 Date: 2008-04-01
MAIN-CHAIN BOND REFINED ATOMS WEIGHT: 2.000 Date: 2008-04-01
MAIN-CHAIN ANGLE REFINED ATOMS COUNT: 7443 Date: 2008-04-01
MAIN-CHAIN ANGLE REFINED ATOMS RMS: 1.287 Date: 2008-04-01
MAIN-CHAIN ANGLE REFINED ATOMS WEIGHT: 3.000 Date: 2008-04-01
SIDE-CHAIN BOND REFINED ATOMS COUNT: 2960 Date: 2008-04-01
SIDE-CHAIN BOND REFINED ATOMS RMS: 0.915 Date: 2008-04-01
SIDE-CHAIN BOND REFINED ATOMS WEIGHT: 2.000 Date: 2008-04-01
SIDE-CHAIN ANGLE REFINED ATOMS COUNT: 2847 Date: 2008-04-01
SIDE-CHAIN ANGLE REFINED ATOMS RMS: 1.369 Date: 2008-04-01
SIDE-CHAIN ANGLE REFINED ATOMS WEIGHT: 3.000 Date: 2008-04-01
METHOD USED: MASK Date: 2008-04-01
VDW PROBE RADIUS: 1.20 Date: 2008-04-01
ION PROBE RADIUS: 0.80 Date: 2008-04-01
SHRINKAGE RADIUS: 0.80 Date: 2008-04-01
Refinement Date : 2008-03-25 Date: 2008-04-01
Refinement Technical Note: qc_check_HP10638A_032508.html Date: 2008-03-25
Structure Refinement Summary: HP10638A_Xtal55064_032508.doc Date: 2008-03-25
Matthews Coef: 3.36 Date: 2009-07-26
Solvent Percentage: 63.42 Date: 2009-07-26

STAGE: QC

Collection: 60024 Cell: 1 Date: 2008-03-17 Status: QC Return Previous Location: Collection: 59465 Cell: 1

Performed By: 2793 Date: 2008-03-17
QC Log: qc1 Date: 2008-03-17
QC Date: 2008-03-17 Date: 2008-03-17
QC Set Name: qc1 Date: 2008-03-17

Collection: 60024 Cell: 2 Date: 2008-03-26 Status: Success Previous Location: Collection: 59465 Cell: 2

QC Comments: ready for deposition Date: 2008-03-26
Performed By: 2793 Date: 2008-03-26
QC Date: 2008-03-26 Date: 2008-03-26
QC Set Name: qc2 Date: 2008-03-26

STAGE: Validation

Collection: 60350 Cell: 1 Date: 2008-04-01 Status: Success Previous Location: Collection: 60024 Cell: 2

STAGE: PDB Deposition

Collection: 60464 Cell: 1 Date: 2008-04-01 Status: Success Previous Location: Collection: 60350 Cell: 1

PDB ID: 3cpx Date: 2008-04-10
RCSB ID: rcsb047069 Date: 2008-04-10
PDB File: C--Documents-and-Settings-tamara-Desktop-PDB-DEPOSITION-HP10638A-redo-hp10638a.pdb Date: 2008-04-10
Coordinate mmCIF File: C--Documents-and-Settings-tamara-Desktop-PDB-DEPOSITION-HP10638A-redo-hp10638a_coor.mmCIF.gz Date: 2008-04-10
Structure factor mmCIF File: C--Documents-and-Settings-tamara-Desktop-PDB-DEPOSITION-HP10638A-redo-hp10638a_sf.mmCIF.gz Date: 2008-04-10
Deposition Date: 2008-04-01 Date: 2008-04-10

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