| Target ID: | 375102 | ||||||||||||
| Target Name: | HP10638A | Protein Accession: | YP_676701.1 | Local Accession: | HP10638A | cDNA Accession: | REF|NC_008255.1|:80866-81774 | PSCA | PDB:3cpx |
| Hover over nodes to see experimental data. Click on nodes to go to that point in the data below. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Name: | putative M42 glutamyl aminopeptidase | Start Position: | 1 | End Position: | 302 | ||
| GNF Target Alias: | HK100:SpeedET-HP10638A-chu-302-1-302 | Sequence Type: | COMPLETE | Residues: | 302 | ||
| DNA Length: | 906 | Molecular Weight: | 34277.17 | Isoelectric Point: | 4.90 | ||
| Epsilon 280: | 61880 | Gravy Index: | -0.26 | Species: | C. hutchinsonii ATCC 33406 | ||
| Activated: | 1 | Alanine Absolute: | 18 | Alanine Relative: | 0.0596 | ||
| Arginine Absolute: | 14 | Arginine Relative: | 0.0464 | Asparagine Absolute: | 9 | ||
| Asparagine Relative: | 0.0298 | Aspartic Acid Absolute: | 21 | Aspartic Acid Relative: | 0.0695 | ||
| Cysteine Absolute: | 6 | Cysteine Relative: | 0.0199 | Glutamic Acid Absolute: | 27 | ||
| Glutamic Acid Relative: | 0.0894 | Glutamine Absolute: | 7 | Glutamine Relative: | 0.0232 | ||
| Glycine Absolute: | 24 | Glycine Relative: | 0.0795 | Histidine Absolute: | 9 | ||
| Histidine Relative: | 0.0298 | Isoleucine Absolute: | 23 | Isoleucine Relative: | 0.0762 | ||
| Leucine Absolute: | 27 | Leucine Relative: | 0.0894 | Lysine Absolute: | 16 | ||
| Lysine Relative: | 0.0530 | Methionine Absolute: | 7 | Methionine Relative: | 0.0232 | ||
| Phenylalanine Absolute: | 11 | Phenylalanine Relative: | 0.0364 | Proline Absolute: | 14 | ||
| Proline Relative: | 0.0464 | Serine Absolute: | 16 | Serine Relative: | 0.0530 | ||
| Threonine Absolute: | 14 | Threonine Relative: | 0.0464 | Tryptophan Absolute: | 8 | ||
| Tryptophan Relative: | 0.0265 | Tyrosine Absolute: | 12 | Tyrosine Relative: | 0.0397 | ||
| Valine Absolute: | 19 | Valine Relative: | 0.0629 | Project: | Zn peptidases | ||
| Selection Date: | 2007-02-27 |
STAGE: Proposed Target
STAGE: Active Target
-
Plate: 45932 Cell: H04 Date: 2007-03-22 Status: Success
Previous Location: Plate: 0 Cell: 375102
- Sample Name: HK100:SpeedET-HP10638A-chu-302-1-302 Date: 2008-10-19
- GNF Project Name: PSI2_LargePFAMproj5_230(FB-HP-RK) Date: 2008-10-19
- GNF Program Name: JCSG Date: 2008-10-19
STAGE: PCR Testing
STAGE: Amplifer Generation
STAGE: Orientation
STAGE: Plasmid Purification
-
Plate: 45932 (C-0475) Cell: H04 Date: 2007-03-22 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Clone ID: 32169 Date: 2007-03-24
- Strain/Vector: HK100 Date: 2008-10-19
- Clone Site: SpeedET Date: 2008-10-19
- Cloned Date: 2007-03-22 Date: 2008-10-19
STAGE: Micro Expression
-
Plate: 45992 Cell: H04 Date: 2007-03-23 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
-
Plate: 59469 Cell: C07 Date: 2008-02-12 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
-
Plate: 60392 Cell: C07 Date: 2008-03-12 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
STAGE: Expression
-
Plate: 47552 (E-1115) Cell: A05 Date: 2007-04-25 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Comment: His-tag's on Date: 2008-10-19
- Fermentation Date: 2007-04-25 Date: 2008-10-19
- GNFuge Date: 2007-11-29 Date: 2008-10-19
- Protein ID: 99513 Date: 2007-05-08
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 47552 (E-1115) Cell: B05 Date: 2007-04-25 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2007-04-25 Date: 2008-10-19
- GNFuge Date: 2007-11-29 Date: 2008-10-19
- Protein ID: 99525 Date: 2007-05-08
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 47552 (E-1115) Cell: C05 Date: 2007-04-25 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2007-04-25 Date: 2008-10-19
- GNFuge Date: 2007-11-29 Date: 2008-10-19
- Protein ID: 99537 Date: 2007-05-08
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 47552 (E-1115) Cell: D05 Date: 2007-04-25 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2007-04-25 Date: 2008-10-19
- GNFuge Date: 2007-11-29 Date: 2008-10-19
- Protein ID: 99549 Date: 2007-05-08
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 47552 (E-1115) Cell: E05 Date: 2007-04-25 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2007-04-25 Date: 2008-10-19
- GNFuge Date: 2007-11-29 Date: 2008-10-19
- Protein ID: 99561 Date: 2007-05-08
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 47552 (E-1115) Cell: F05 Date: 2007-04-25 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2007-04-25 Date: 2008-10-19
- GNFuge Date: 2007-11-29 Date: 2008-10-19
- Protein ID: 99573 Date: 2007-05-08
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 47552 (E-1115) Cell: G05 Date: 2007-04-25 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2007-04-25 Date: 2008-10-19
- GNFuge Date: 2007-11-29 Date: 2008-10-19
- Protein ID: 99585 Date: 2007-05-08
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 47552 (E-1115) Cell: H05 Date: 2007-04-25 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2007-04-25 Date: 2008-10-19
- GNFuge Date: 2007-11-29 Date: 2008-10-19
- Protein ID: 99597 Date: 2007-05-08
- Media: Native-TB Date: 2008-10-19
-
Plate: 62193 (E-1442) Cell: A08 Date: 2008-05-15 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Comment: His-tag's on Date: 2008-10-19
- Fermentation Date: 2008-05-15 Date: 2008-10-19
- Protein ID: 134398 Date: 2008-05-17
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 62193 (E-1442) Cell: B08 Date: 2008-05-15 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-05-15 Date: 2008-10-19
- Protein ID: 134410 Date: 2008-05-17
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 62193 (E-1442) Cell: C08 Date: 2008-05-15 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-05-15 Date: 2008-10-19
- Protein ID: 134422 Date: 2008-05-17
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 62193 (E-1442) Cell: D08 Date: 2008-05-15 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-05-15 Date: 2008-10-19
- Protein ID: 134434 Date: 2008-05-17
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 62193 (E-1442) Cell: E08 Date: 2008-05-15 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-05-15 Date: 2008-10-19
- Protein ID: 134446 Date: 2008-05-17
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 62193 (E-1442) Cell: F08 Date: 2008-05-15 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-05-15 Date: 2008-10-19
- Protein ID: 134458 Date: 2008-05-17
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 62193 (E-1442) Cell: G08 Date: 2008-05-15 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-05-15 Date: 2008-10-19
- Protein ID: 134470 Date: 2008-05-17
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 62193 (E-1442) Cell: H08 Date: 2008-05-15 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-05-15 Date: 2008-10-19
- Protein ID: 134482 Date: 2008-05-17
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 64591 (E-1507) Cell: F05 Date: 2008-07-08 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-07-08 Date: 2010-07-14
- Protein ID: 141470 Date: 2008-07-10
- Media: Native-TB Date: 2008-10-19
-
Plate: 64591 (E-1507) Cell: F06 Date: 2008-07-08 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-07-08 Date: 2010-07-14
- Protein ID: 141471 Date: 2008-07-10
- Media: Native-TB Date: 2008-10-19
-
Plate: 66815 (E-1575) Cell: A01 Date: 2008-08-27 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-08-28 Date: 2009-06-18
- GNFuge Date: 2008-11-11 Date: 2009-06-18
- Protein ID: 148953 Date: 2008-08-27
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 66815 (E-1575) Cell: B01 Date: 2008-08-27 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-08-28 Date: 2009-06-18
- GNFuge Date: 2008-11-11 Date: 2009-06-18
- Protein ID: 148965 Date: 2008-08-27
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 66815 (E-1575) Cell: C01 Date: 2008-08-27 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-08-28 Date: 2009-06-18
- GNFuge Date: 2008-11-11 Date: 2009-06-18
- Protein ID: 148977 Date: 2008-08-27
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 66815 (E-1575) Cell: D01 Date: 2008-08-27 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-08-28 Date: 2009-06-18
- GNFuge Date: 2008-11-11 Date: 2009-06-18
- Protein ID: 148989 Date: 2008-08-27
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 66815 (E-1575) Cell: E01 Date: 2008-08-27 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-08-28 Date: 2009-06-18
- GNFuge Date: 2008-11-11 Date: 2009-06-18
- Protein ID: 149001 Date: 2008-08-27
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 66815 (E-1575) Cell: F01 Date: 2008-08-27 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-08-28 Date: 2009-06-18
- GNFuge Date: 2008-11-11 Date: 2009-06-18
- Protein ID: 149013 Date: 2008-08-27
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 66815 (E-1575) Cell: G01 Date: 2008-08-27 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-08-28 Date: 2009-06-18
- GNFuge Date: 2008-11-11 Date: 2009-06-18
- Protein ID: 149025 Date: 2008-08-27
- Media: Met-Inhibition Date: 2008-10-19
-
Plate: 66815 (E-1575) Cell: H01 Date: 2008-08-27 Status: Success
Previous Location: Plate: 45932 (C-0475) Cell: H04
- Fermentation Date: 2008-08-28 Date: 2009-06-18
- GNFuge Date: 2008-11-11 Date: 2009-06-18
- Protein ID: 149037 Date: 2008-08-27
- Media: Met-Inhibit