PSCA sequence

YP_807469.1;PE00025C	Protein Sequence Comparative Analysis (PSCA)

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YP_807469.1 Chemical properties of protein sequence with tag

Accession: YP_807469.1
Description: gi|116495735|ref|YP_807469.1| hypothetical protein LSEI_2283 [Lactobacillus casei ATCC 334]
Organism: LACTOBACILLUS CASEI ATCC 334

	No Tag	Tag
Residues(aa)	122	134
Molecular Weight (Dalton)	14434.85	15889.40
Isoelectric Point	6.5	6.83
Extinction Coefficient	26470	26470
Gravy	-.52	-0.64
Number of Met	5	6
%Met	4.10	4.48
Number of Cys	0	0
%Cys	0.00	0.00

SEQUENCE  amino acids 134 aa; containing Tag mgsdkihhhhhh
>YP_807469.1 gi|116495735|ref|YP_807469.1| hypothetical protein LSEI_2283 [Lactobacillus casei ATCC 334]
mgsdkihhhhhhMGSLAEWYQRIPTPDDLTRVESLFANMQAQFPQLKLEFKWNQPMFTDH
GTFIMGFNPSKKHLAVAIEPQTMTRFIPQIDKAGYDHSQIIRFPWHKPLDEQLIHDLIAY
TIDQKKDATTFWQR

Notes: Most of the JCSG constructs have a 6thio6his tag immediately before the N-terminal tag. This means that every protein expressed and purified has the tag amino acids (MGSDKIHHHHHH) in front of the N-terminus.

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