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   TM0010;TM0010
 Protein Sequence Comparative Analysis   (PSCA)
 
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 TM0010 Chemical properties of protein sequence with tag

Accession: TM0010
Description: TM0010 NADP-reducing hydrogenase, subunit C (hndC-1)
Organism: THERMOTOGA MARITIMA
  No Tag Tag
Residues(aa) 607 619
Molecular Weight (Dalton) 66077.85 67532.40
Isoelectric Point 5.41 5.68
Extinction Coefficient 44810 44810
Gravy -.09 -0.12
Number of Met 13 14
%Met 2.14 2.26
Number of Cys 23 23
%Cys 3.79 3.72 
SEQUENCE  amino acids 619 aa; containing Tag mgsdkihhhhhh
>TM0010 TM0010 NADP-reducing hydrogenase, subunit C (hndC-1)
mgsdkihhhhhhMPLTTNTILICAGGACISAGEKSVKDAFEEELRKYGLDEVVRVIETGC
MGACTLGPIAVIYPESVFYQKLTPDAAREIVEEHILKGRIVEKYLYKASEGKPVPRVHEE
VPFFKKQVRIVTRNLGVIDPTKIEEYIARDGYFALAKALQMKPEDVIKEIKDSGLRGRGG
AGFPTGLKWELAARQKAEKKFVVCNADEGDPGAFMDRSVLEGDPHAVIEGMAIAAYAIGA
QKGFVYVRAEYPLAIERLNIALNQAREYGFLGGNIMGTDFSFDIEIRIGAGAFVCGEETA
LMASIEGERGQPKVKPPYPVEKGLWGYPTVINNVETLANVPWIIRNGAKEFRKYGTENSP
GTKVFALAGKVKNTGLVEVPMGITLRELIYEIGGGIAGDKKLKAIQTGGPSGGCIPAEYV
DTPVDFESLQKLGAIMGSGGMIVMDEDDCMVDVARFFLEFTVEESCGKCTPCREGTKKML
EILEKITSGEGTEEDIEELEKLAHVVKDTSLCGLGQTAPNPVLSTLRYFRDEYLAHVKEK
RCPSKKCKALISYVIDPEKCVGCTACARVCPVQCISGQVRQPHVIDQAECVRCGSCIEVC
RFGAISKVTPALPVEEAVE
Notes: Most of the JCSG constructs have a 6thio6his tag immediately before the N-terminal tag. This means that every protein expressed and purified has the tag amino acids (MGSDKIHHHHHH) in front of the N-terminus.
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