PSCA sequence

TM0012;TM0012	Protein Sequence Comparative Analysis (PSCA)

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TM0012 Chemical properties of target construct

Accession: TM0012
Description: TM0012 NADP-reducing hydrogenase, subunit A (hndA-1)
Organism: THERMOTOGA MARITIMA
Residues: 176 aa
Local acc: TM0012
Target id: 281893
Alias:
Construct: Full: 1-176
Warning: the change of start codon from ATG/M to GTG/V;

	Full	Fragment	Fragment with tag	Fragment with modification	Fragment with tag and modification
Residues(aa)	176	176	188	176	188
Molecular Weight (Dalton)	19957.02	19957.02	21411.57	19924.96	21379.51
Isoelectric Point	5.32	5.32	5.98	5.32	5.98
Extinction Coefficient	11920	11920	11920	11920	11920
Gravy	-0.24	-0.24	-0.34	-0.22	-0.32
Number of Met	5	5	6	4	5
%Met	2.84	2.84	3.19	2.27	2.66
Number of Cys	4	4	4	4	4
%Cys	>2.27	2.27	2.13	2.27	2.13

SEQUENCE  amino acids 188 aa; construct sequence with modification and tag mgsdkihhhhhh
>TM0012|1-176 TM0012 NADP-reducing hydrogenase, subunit A (hndA-1)
mgsdkihhhhhhVKKFPINSDQEVAVLVKSKEELFKELENFIEENGYEGKKDALIQVLHK
AQELFGYLPADVLEYISDKLDVPLSKVYGVVTFYNFFSTKPKGKHQIKVCLGTACYVKGA
DRIFERFLEELKVNPDEPTSDGMFSVHGVRCLGACSMAPVVMVDEDDFYGRVTPDMVPQI
ISKYKREG

SEQUENCE  amino acids 176 aa; construct sequence without modification or tag
>TM0012|1-176 TM0012 NADP-reducing hydrogenase, subunit A (hndA-1)
MKKFPINSDQEVAVLVKSKEELFKELENFIEENGYEGKKDALIQVLHKAQELFGYLPADV
LEYISDKLDVPLSKVYGVVTFYNFFSTKPKGKHQIKVCLGTACYVKGADRIFERFLEELK
VNPDEPTSDGMFSVHGVRCLGACSMAPVVMVDEDDFYGRVTPDMVPQIISKYKREG

SEQUENCE  amino acids 188 aa; construct sequence with tag but no modification; mgsdkihhhhhh
>TM0012|1-176 TM0012 NADP-reducing hydrogenase, subunit A (hndA-1)
mgsdkihhhhhhMKKFPINSDQEVAVLVKSKEELFKELENFIEENGYEGKKDALIQVLHK
AQELFGYLPADVLEYISDKLDVPLSKVYGVVTFYNFFSTKPKGKHQIKVCLGTACYVKGA
DRIFERFLEELKVNPDEPTSDGMFSVHGVRCLGACSMAPVVMVDEDDFYGRVTPDMVPQI
ISKYKREG

SEQUENCE  amino acids 176 aa; construct sequence with modification but no tag
>TM0012|1-176 TM0012 NADP-reducing hydrogenase, subunit A (hndA-1)
VKKFPINSDQEVAVLVKSKEELFKELENFIEENGYEGKKDALIQVLHKAQELFGYLPADV
LEYISDKLDVPLSKVYGVVTFYNFFSTKPKGKHQIKVCLGTACYVKGADRIFERFLEELK
VNPDEPTSDGMFSVHGVRCLGACSMAPVVMVDEDDFYGRVTPDMVPQIISKYKREG

SEQUENCE  amino acids 176 aa; full sequence in database
>TM0012|1-176 TM0012 NADP-reducing hydrogenase, subunit A (hndA-1)
MKKFPINSDQEVAVLVKSKEELFKELENFIEENGYEGKKDALIQVLHKAQELFGYLPADV
LEYISDKLDVPLSKVYGVVTFYNFFSTKPKGKHQIKVCLGTACYVKGADRIFERFLEELK
VNPDEPTSDGMFSVHGVRCLGACSMAPVVMVDEDDFYGRVTPDMVPQIISKYKREG

Notes: Most of the JCSG constructs have a 6thio6his tag immediately before the N-terminal tag. This means that every protein expressed and purified has the tag amino acids (MGSDKIHHHHHH) in front of the N-terminus. Special tag in protocol 30: N-terminal HHHHHHTDPALRA, first residue deleted and C-terminal GLCGR.

The start codon is a ATG/Methionine in most of sequences, but the GTG/V, TTG/L, CTG/I can be the start codons in some cases as expressing in E.coli. The start codon warning is labelled by RED color in sequence(sample: 10174951).

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